Title :

Enhancing Colistin Activity against Colistin-Resistant Escherichia coli through Combination with Alginate Nanoparticles and Small Molecules

Author(s) :

Hazime, Noura [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Belguesmia, Yanath [Auteur]
BioEcoAgro - Equipe 8 - Food and Digestive Microbial Ecosystems: Interactions - Dynamics - Application(s)
Kempf, Isabelle [Auteur]
Laboratoire de Ploufragan-Plouzané-Niort [ANSES]
Barras, Alexandre [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
NanoBioInterfaces - IEMN [NBI - IEMN]
Drider, Djamel [Auteur correspondant]
BioEcoAgro - Equipe 8 - Food and Digestive Microbial Ecosystems: Interactions - Dynamics - Application(s)
Boukherroub, Rabah [Auteur correspondant]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
NanoBioInterfaces - IEMN [NBI - IEMN]

Journal title :

Pharmaceuticals

Pages :

682

Publisher :

MDPI

Publication date :

2022-06

ISSN :

1424-8247

English keyword(s) :

antimicrobial activity
colistin
essential oils
polyamines
lactic acid
Escherichia coli
alginate nanoparticles

HAL domain(s) :

Chimie/Polymères
Chimie/Chimie thérapeutique
Sciences du Vivant [q-bio]/Sciences pharmaceutiques

English abstract : [en]

Bacterial resistance to antibiotics has become a major public health problem worldwide, with the yearly number of deaths exceeding 700,000. To face this well-acknowledged threat, new molecules and therapeutic methods are ...
Show more >Bacterial resistance to antibiotics has become a major public health problem worldwide, with the yearly number of deaths exceeding 700,000. To face this well-acknowledged threat, new molecules and therapeutic methods are considered. In this context, the application of nanotechnology to fight bacterial infection represents a viable approach and has experienced tremendous developments in the last decades. Escherichia coli (E. coli) is responsible for severe diarrhea, notably in the breeding sector, and especially in pig farming. The resulting infection (named colibacillosis) occurs in young piglets and could lead to important economic losses. Here, we report the design of several new formulations based on colistin loaded on alginate nanoparticles (Alg NPs) in the absence, but also in the presence, of small molecules, such as components of essential oils, polyamines, and lactic acid. These new formulations, which are made by concomitantly binding colistin and small molecules to Alg NPs, were successfully tested against E. coli 184, a strain resistant to colistin. When colistin was associated with Alg NPs, the minimal inhibition concentration (MIC) decreased from 8 to 1 µg/mL. It is notable that when menthol or lactic acid was co-loaded with colistin on Alg NPs, the MIC of colistin drastically decreased, reaching 0.31 or 0.62 µg/mL, respectively. These novel bactericidal formulations, whose innocuity towards eukaryotic HT-29 cells was established in vitro, are presumed to permeabilize the bacterial membrane and provoke the leakage of intracellular proteins. Our findings revealed the potentiating effect of the Alg NPs on colistin, but also of the small molecules mentioned above. Such ecological and economical formulations are easy to produce and could be proposed, after confirmation by in vivo and toxicology tests, as therapeutic strategies to replace fading antibiotics.Show less >

Language :

Anglais

Popular science :

Non

ANR Project :

Alternatives stratégiques pour réduire l'usage de la colistine dans les pratiques d'élevage de porc

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520

Source :

Harvested from HAL