Title :

S-ship promoter expression identifies mouse mammary cancer stem cells

Author(s) :

Tian, Lu [Auteur]
Truong, Marie-Jose [Auteur]
Lagadec, Chann [Auteur]
Plasticité Cellulaire et Cancer (CPAC) - U908
Adriaenssens, Eric [Auteur]
Plasticité Cellulaire et Cancer (CPAC) - U908
Bouchaert, Emmanuel [Auteur]
Bauderlique-Le Roy, Helene [Auteur]
Figeac, Martin [Auteur]
Le Bourhis, Xuefen [Auteur]
Plasticité Cellulaire et Cancer (CPAC) - U908
Bourette, Roland P. [Auteur]

Journal title :

Stem cell reports

Abbreviated title :

Stem Cell Reports

Publication date :

2019-05-28

ISSN :

2213-6711

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

During normal mammary gland development, s-SHIP promoter expression marks a distinct type of mammary stem cells, at two different stages, puberty and early mid-pregnancy. To determine whether s-SHIP is a marker of mammary ...
Show more >During normal mammary gland development, s-SHIP promoter expression marks a distinct type of mammary stem cells, at two different stages, puberty and early mid-pregnancy. To determine whether s-SHIP is a marker of mammary cancer stem cells (CSCs), we generated bitransgenic mice by crossing the C3(1)-SV40 T-antigen transgenic mouse model of breast cancer, and a transgenic mouse (11.5kb-GFP) expressing green fluorescent protein from the s-SHIP promoter. Here we show that in mammary tumors originating in these bitransgenic mice, s-SHIP promoter expression enriches a rare cell population with CSC activity as demonstrated by sphere-forming assays in vitro and limiting dilution transplantation in vivo. These s-SHIP-positive CSCs are characterized by lower expression of Delta-like non-canonical Notch ligand 1 (DLK1), a negative regulator of the Notch pathway. Inactivation of Dlk1 in s-SHIP-negative tumor cells increases their tumorigenic potential, suggesting a role for DLK1 in mammary cancer stemness.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CNRS
INSERM
Université de Lille

Collections :

• Miniaturisation pour la Synthèse, l'Analyse et la Protéomique (MSAP) - USR 3290
• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Submission date :

2022-06-15T13:57:44Z