S-ship promoter expression identifies mouse ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
S-ship promoter expression identifies mouse mammary cancer stem cells
Auteur(s) :
Tian, Lu [Auteur]
Truong, Marie-Jose [Auteur]
Lagadec, Chann [Auteur]
Plasticité Cellulaire et Cancer (CPAC) - U908
Adriaenssens, Eric [Auteur]
Plasticité Cellulaire et Cancer (CPAC) - U908
Bouchaert, Emmanuel [Auteur]
Bauderlique-Le Roy, Helene [Auteur]
Figeac, Martin [Auteur]
Le Bourhis, Xuefen [Auteur]
Plasticité Cellulaire et Cancer (CPAC) - U908
Bourette, Roland P. [Auteur]
Truong, Marie-Jose [Auteur]
Lagadec, Chann [Auteur]
Plasticité Cellulaire et Cancer (CPAC) - U908
Adriaenssens, Eric [Auteur]
Plasticité Cellulaire et Cancer (CPAC) - U908
Bouchaert, Emmanuel [Auteur]
Bauderlique-Le Roy, Helene [Auteur]
Figeac, Martin [Auteur]
Le Bourhis, Xuefen [Auteur]
Plasticité Cellulaire et Cancer (CPAC) - U908
Bourette, Roland P. [Auteur]
Titre de la revue :
Stem cell reports
Nom court de la revue :
Stem Cell Reports
Date de publication :
2019-05-28
ISSN :
2213-6711
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
During normal mammary gland development, s-SHIP promoter expression marks a distinct type of mammary stem cells, at two different stages, puberty and early mid-pregnancy. To determine whether s-SHIP is a marker of mammary ...
Lire la suite >During normal mammary gland development, s-SHIP promoter expression marks a distinct type of mammary stem cells, at two different stages, puberty and early mid-pregnancy. To determine whether s-SHIP is a marker of mammary cancer stem cells (CSCs), we generated bitransgenic mice by crossing the C3(1)-SV40 T-antigen transgenic mouse model of breast cancer, and a transgenic mouse (11.5kb-GFP) expressing green fluorescent protein from the s-SHIP promoter. Here we show that in mammary tumors originating in these bitransgenic mice, s-SHIP promoter expression enriches a rare cell population with CSC activity as demonstrated by sphere-forming assays in vitro and limiting dilution transplantation in vivo. These s-SHIP-positive CSCs are characterized by lower expression of Delta-like non-canonical Notch ligand 1 (DLK1), a negative regulator of the Notch pathway. Inactivation of Dlk1 in s-SHIP-negative tumor cells increases their tumorigenic potential, suggesting a role for DLK1 in mammary cancer stemness.Lire moins >
Lire la suite >During normal mammary gland development, s-SHIP promoter expression marks a distinct type of mammary stem cells, at two different stages, puberty and early mid-pregnancy. To determine whether s-SHIP is a marker of mammary cancer stem cells (CSCs), we generated bitransgenic mice by crossing the C3(1)-SV40 T-antigen transgenic mouse model of breast cancer, and a transgenic mouse (11.5kb-GFP) expressing green fluorescent protein from the s-SHIP promoter. Here we show that in mammary tumors originating in these bitransgenic mice, s-SHIP promoter expression enriches a rare cell population with CSC activity as demonstrated by sphere-forming assays in vitro and limiting dilution transplantation in vivo. These s-SHIP-positive CSCs are characterized by lower expression of Delta-like non-canonical Notch ligand 1 (DLK1), a negative regulator of the Notch pathway. Inactivation of Dlk1 in s-SHIP-negative tumor cells increases their tumorigenic potential, suggesting a role for DLK1 in mammary cancer stemness.Lire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
CNRS
INSERM
Université de Lille
INSERM
Université de Lille
Collections :
Date de dépôt :
2022-06-15T13:57:44Z