Universal sample preparation unlocking ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
Universal sample preparation unlocking multimodal molecular tissue imaging
Auteur(s) :
Dannhorn, Andreas [Auteur]
Kazanc, Emine [Auteur]
Ling, Stephanie [Auteur]
Nikula, Chelsea J. [Auteur]
Karali, Evdoxia [Auteur]
Serra, Maria Paola [Auteur]
Vorng, Jean-Luc [Auteur]
Inglese, Paolo [Auteur]
Maglennon, Gareth [Auteur]
Hamm, Gregory [Auteur]
Swales, John [Auteur]
Strittmatter, Nicole [Auteur]
Barry, Simon T. [Auteur]
Sansom, Owen J. [Auteur]
Poulogiannis, George [Auteur]
Bunch, Josephine [Auteur]
Goodwin, Richard J. A. [Auteur]
Takats, Zoltan [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Kazanc, Emine [Auteur]
Ling, Stephanie [Auteur]
Nikula, Chelsea J. [Auteur]
Karali, Evdoxia [Auteur]
Serra, Maria Paola [Auteur]
Vorng, Jean-Luc [Auteur]
Inglese, Paolo [Auteur]
Maglennon, Gareth [Auteur]
Hamm, Gregory [Auteur]
Swales, John [Auteur]
Strittmatter, Nicole [Auteur]
Barry, Simon T. [Auteur]
Sansom, Owen J. [Auteur]
Poulogiannis, George [Auteur]
Bunch, Josephine [Auteur]
Goodwin, Richard J. A. [Auteur]
Takats, Zoltan [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Titre de la revue :
Analytical Chemistry
Nom court de la revue :
Anal. Chem.
Numéro :
92
Pagination :
-
Date de publication :
2020-08-18
ISSN :
0003-2700
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
A new tissue sample embedding and processing method is presented that provides downstream compatibility with numerous different histological, molecular biology, and analytical techniques. The methodology is based on the ...
Lire la suite >A new tissue sample embedding and processing method is presented that provides downstream compatibility with numerous different histological, molecular biology, and analytical techniques. The methodology is based on the low temperature embedding of fresh frozen specimens into a hydrogel matrix composed of hydroxypropyl methylcellulose (HPMC) and polyvinylpyrrolidone (PVP) and sectioning using a cryomicrotome. The hydrogel was expected not to interfere with standard tissue characterization methods, histologically or analytically. We assessed the compatibility of this protocol with various mass spectrometric imaging methods including matrix-assisted laser desorption ionization (MALDI), desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS). We also demonstrated the suitability of the universal protocol for extraction based molecular biology techniques such as rt-PCR. The integration of multiple analytical modalities through this universal sample preparation protocol offers the ability to study tissues at a systems biology level and directly linking results to tissue morphology and cellular phenotype.Lire moins >
Lire la suite >A new tissue sample embedding and processing method is presented that provides downstream compatibility with numerous different histological, molecular biology, and analytical techniques. The methodology is based on the low temperature embedding of fresh frozen specimens into a hydrogel matrix composed of hydroxypropyl methylcellulose (HPMC) and polyvinylpyrrolidone (PVP) and sectioning using a cryomicrotome. The hydrogel was expected not to interfere with standard tissue characterization methods, histologically or analytically. We assessed the compatibility of this protocol with various mass spectrometric imaging methods including matrix-assisted laser desorption ionization (MALDI), desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS). We also demonstrated the suitability of the universal protocol for extraction based molecular biology techniques such as rt-PCR. The integration of multiple analytical modalities through this universal sample preparation protocol offers the ability to study tissues at a systems biology level and directly linking results to tissue morphology and cellular phenotype.Lire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
INSERM
Université de Lille
Université de Lille
Date de dépôt :
2022-06-15T13:57:54Z