Title :

The intelligent knife (iknife) and its intraoperative diagnostic advantage for the treatment of cervical disease

Author(s) :

Tzafetas, Menelaos [Auteur]
Mitra, Anita [Auteur]
Paraskevaidi, Maria [Auteur]
Bodai, Zsolt [Auteur]
Kalliala, Ilkka [Auteur]
Bowden, Sarah [Auteur]
Lathouras, Konstantinos [Auteur]
Rosini, Francesca [Auteur]
Szasz, Marcell [Auteur]
Savage, Adele [Auteur]
Balog, Julia [Auteur]
Mckenzie, James S. [Auteur]
Lyons, Deirdre [Auteur]
Bennett, Phillip R. [Auteur]
Macintyre, David A. [Auteur]
Ghaem-Maghami, Sadaf [Auteur]
Takats, Zoltan [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Kyrgiou, Maria [Auteur]

Journal title :

Proceedings of the National Academy of Sciences of the United States of America

Abbreviated title :

Proc. Natl. Acad. Sci. U. S. A.

Volume number :

117

Pages :

-

Publication date :

2020-03-31

ISSN :

0027-8424

Keyword(s) :

mass spectrometry
REIMS
fertility preservation
iKnife
cervical cancer

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Clearance of surgical margins in cervical cancer prevents the need for adjuvant chemoradiation and allows fertility preservation. In this study, we determined the capacity of the rapid evaporative ionization mass spectrometry ...
Show more >Clearance of surgical margins in cervical cancer prevents the need for adjuvant chemoradiation and allows fertility preservation. In this study, we determined the capacity of the rapid evaporative ionization mass spectrometry (REIMS), also known as intelligent knife (iKnife), to discriminate between healthy, preinvasive, and invasive cervical tissue. Cervical tissue samples were collected from women with healthy, human papilloma virus (HPV) +/- cervical intraepithelial neoplasia (CIN), or cervical cancer. A handheld diathermy device generated surgical aerosol, which was transferred into a mass spectrometer for subsequent chemical analysis. Combination of principal component and linear discriminant analysis and least absolute shrinkage and selection operator was employed to study the spectral differences between groups. Significance of discriminatory m/z features was tested using univariate statistics and tandem MS performed to elucidate the structure of the significant peaks allowing separation of the two classes. We analyzed 87 samples (normal = 16, HPV +/- CIN = 50, cancer = 21 patients). The iKnife discriminated with 100% accuracy normal (100%) vs. HPV +/- CIN (100%) vs. cancer (100%) when compared to histology as the gold standard. When comparing normal vs. cancer samples, the accuracy was 100% with a sensitivity of 100% (95% CI 83.9 to 100) and specificity 100% (79.4 to 100). Univariate analysis revealed significant MS peaks in the cancer-to-normal separation belonging to various classes of complex lipids. The iKnife discriminates healthy from premalignant and invasive cervical lesions with high accuracy and can improve oncological outcomes and fertility preservation of women treated surgically for cervical cancer. Larger in vivo research cohorts are required to validate these findings.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

INSERM
Université de Lille

Collections :

• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Submission date :

2022-06-15T13:59:27Z