Title :

Experimental evidence that polystyrene nanoplastics cross the intestinal barrier of European seabass

Author(s) :

Vagner, Marie [Auteur correspondant]
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Boudry, Gaëlle [Auteur]
Nutrition, Métabolismes et Cancer [NuMeCan]
Courcot, Lucie [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Vincent, Dorothée [Auteur]
Office français de la biodiversité [OFB]
Dehaut, Alexandre [Auteur]
Laboratoire de sécurité des aliments de Maisons-Alfort [LSAl]
Duflos, Guillaume [Auteur]
Laboratoire de sécurité des aliments de Maisons-Alfort [LSAl]
Huvet, Arnaud [Auteur]
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Tallec, Kévin [Auteur]
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]
Zambonino-Infante, Jose-Luis [Auteur]
Laboratoire des Sciences de l'Environnement Marin (LEMAR) [LEMAR]

Journal title :

Environment International

Pages :

107340

Publisher :

Elsevier

Publication date :

2022-08-01

ISSN :

0160-4120

English keyword(s) :

polystyrene nanoplastic beads
translocation
intestinal epithelium
Dicentrarchus labrax
Ussing chambers

HAL domain(s) :

Sciences du Vivant [q-bio]/Toxicologie/Ecotoxicologie
Sciences de l'environnement/Biodiversité et Ecologie
Planète et Univers [physics]/Sciences de la Terre/Océanographie

English abstract : [en]

Plastic pollution in marine ecosystems constitutes an important threat to marine life. For vertebrates, macro/microplastics can obstruct and/or transit into the airways and digestive tract whereas nanoplastics (NPs; < 1000 ...
Show more >Plastic pollution in marine ecosystems constitutes an important threat to marine life. For vertebrates, macro/microplastics can obstruct and/or transit into the airways and digestive tract whereas nanoplastics (NPs; < 1000 nm) have been observed in non-digestive tissues such as the liver and brain. Whether NPs cross the intestinal epithelium to gain access to the blood and internal organs remains controversial, however. Here, we show directly NP translocation across the intestinal barrier of a fish, the European seabass, Dicentrarchus labrax, ex vivo. The luminal side of median and distal segments of intestine were exposed to fluorescent polystyrene NPs (PS-NPs) of 50 nm diameter. PS-NPs that translocated to the serosal side were then detected quantitatively by fluorimetry, and qualitatively by scanning electron microscopy (SEM) and pyrolysis coupled to gas chromatography and high-resolution mass spectrometry (Py-GC-HRMS). Fluorescence intensity on the serosal side increased 15–90 min after PS-NP addition into the luminal side, suggesting that PS-NPs crossed the intestinal barrier; this was confirmed by both SEM and Py-GC-HRMS. This study thus evidenced conclusively that NPs beads translocate across the intestinal epithelium in this marine vertebrate.Show less >

Language :

Anglais

Popular science :

Non

ANR Project :

Microplastiques, nanoplastiques dans l'environnement marin: caractérisation, impacts et évaluation des risques sanitaires.

Collections :

• Laboratoire d'Océanologie et de Géosciences (LOG) - UMR 8187

Source :

Harvested from HAL