Title :

Fast-SEA ligation: a chemoselective NCL-inspired ligation reaction effective in the nanomolar concentration range

Author(s) :

Grain, Benjamin [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Snella, Benoît [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Vicogne, Jérôme [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Capet, Frédéric [Auteur]
Wiltschi, Birgit [Auteur]
Melnyk, Oleg [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
AGOURIDAS, Vangelis [Auteur]
Centrale Lille
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Conference title :

GFPP 22 / BPGM 5 Joint Meeting of the French and Belgian Peptides and Proteins Societies

Conference organizers(s) :

GFPP / BPGM

City :

Port-Leucate

Country :

France

Start date of the conference :

2022-05-29

Publication date :

2022-06-13

English keyword(s) :

Latency
N.S-Acyl Shift Systems
Non-Native Ligation
Protein Modification
Thioester

HAL domain(s) :

Chimie/Chimie organique

English abstract : [en]

We show that latent oxalyl thioester surrogates are a powerful means to modify peptides and proteins in highly dilute conditions in purified aqueous media or in mixtures as complex as cell lysates. Designed to be shelf-stable ...
Show more >We show that latent oxalyl thioester surrogates are a powerful means to modify peptides and proteins in highly dilute conditions in purified aqueous media or in mixtures as complex as cell lysates. Designed to be shelf-stable reagents, they can be activated on demand to enable ligation reactions with peptide concentrations as low as a few hundred nM at rates approaching 30 M-1 s-1.Show less >

Language :

Anglais

Peer reviewed article :

Non

Audience :

Internationale

Popular science :

Oui

ANR Project :

Incorporation métabolique de thioesters latents hautement réactifs : une solution intégrée pour la production de protéines étiquetées et de biopolymères

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL