Native glycosylation and binding of the ...
Type de document :
Article dans une revue scientifique
PMID :
URL permanente :
Titre :
Native glycosylation and binding of the antidepressant paroxetine in a low-resolution crystal structure of human myeloperoxidase
Auteur(s) :
Krawczyk, Lucas [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Semwal, Shubham [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Soubhye, Jalal [Auteur]
Faculté de Pharmacie [Bruxelles] [ULB]
Lemri Ouadriri, Salma [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Prévost, Martin [Auteur]
Faculté des Sciences [Bruxelles] [ULB]
Van Antwerpen, Pierre [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Roos, Goedele [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Bouckaert, Julie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Semwal, Shubham [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Soubhye, Jalal [Auteur]
Faculté de Pharmacie [Bruxelles] [ULB]
Lemri Ouadriri, Salma [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Prévost, Martin [Auteur]
Faculté des Sciences [Bruxelles] [ULB]
Van Antwerpen, Pierre [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Roos, Goedele [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Bouckaert, Julie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Titre de la revue :
Acta Crystallographica Section D Structural Biology
Numéro :
D78
Pagination :
1099-1109
Éditeur :
International Union of Crystallography (IUCr)
Date de publication :
2022-08-09
ISSN :
2059-7983
Mot(s)-clé(s) en anglais :
human myeloperoxidase
glycosylation
N-glycan refinement
thiocyanate
paroxetine
glycosylation
N-glycan refinement
thiocyanate
paroxetine
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Human myeloperoxidase (MPO) utilizes hydrogen peroxide to oxidize organic compounds and as such plays an essential role in cell-component synthesis, in metabolic and elimination pathways, and in the front-line defence ...
Lire la suite >Human myeloperoxidase (MPO) utilizes hydrogen peroxide to oxidize organic compounds and as such plays an essential role in cell-component synthesis, in metabolic and elimination pathways, and in the front-line defence against pathogens. Moreover, MPO is increasingly being reported to play a role in inflammation. The enzymatic activity of MPO has also been shown to depend on its glycosylation. Mammalian MPO crystal structures deposited in the Protein Data Bank (PDB) present only a partial identification of their glycosylation. Here, a newly obtained crystal structure of MPO containing four disulfide-linked dimers and showing an elaborate collection of glycans is reported. These are compared with the glycans identified in proteomics studies and from 18 human MPO structures available in the PDB. The crystal structure also contains bound paroxetine, a blocker of serotonin reuptake that has previously been identified as an irreversible inhibitor of MPO, in the presence of thiocyanate, a physiological substrate of MPO.Lire moins >
Lire la suite >Human myeloperoxidase (MPO) utilizes hydrogen peroxide to oxidize organic compounds and as such plays an essential role in cell-component synthesis, in metabolic and elimination pathways, and in the front-line defence against pathogens. Moreover, MPO is increasingly being reported to play a role in inflammation. The enzymatic activity of MPO has also been shown to depend on its glycosylation. Mammalian MPO crystal structures deposited in the Protein Data Bank (PDB) present only a partial identification of their glycosylation. Here, a newly obtained crystal structure of MPO containing four disulfide-linked dimers and showing an elaborate collection of glycans is reported. These are compared with the glycans identified in proteomics studies and from 18 human MPO structures available in the PDB. The crystal structure also contains bound paroxetine, a blocker of serotonin reuptake that has previously been identified as an irreversible inhibitor of MPO, in the presence of thiocyanate, a physiological substrate of MPO.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Université de Lille
CNRS
CNRS
Équipe(s) de recherche :
Computational Molecular Systems Biology
Date de dépôt :
2022-09-20T14:26:11Z
2022-09-21T13:39:55Z
2022-10-19T12:46:55Z
2022-09-21T13:39:55Z
2022-10-19T12:46:55Z
Fichiers
- P22.19 MPO_paroxetine_glycosilatie_crystal.pdf
- Version finale acceptée pour publication (postprint)
- Accès libre
- Accéder au document