Integrated sRNA-seq and RNA-seq Analyses ...
Document type :
Article dans une revue scientifique: Article original
DOI :
PMID :
Title :
Integrated sRNA-seq and RNA-seq Analyses Reveal a microRNA Regulation Network Involved in Cold Response in Pisum sativum L.
Author(s) :
Mazurier, Mélanie [Auteur]
Ecotoxicologie & Santé des écosystèmes - ECSECO [ECI]
Drouaud, Jan [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Bahrman, Nasser [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Rau, Andrea [Auteur]
Génétique Animale et Biologie Intégrative [GABI]
Lejeune-Hénaut, Isabelle [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Delbreil, Bruno [Auteur]
BioEcoAgro - Equipe 1 - Functioning and adaptation of the plant in interaction with its environment
Legrand, Sylvain [Auteur correspondant]
Université de Lille
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Ecotoxicologie & Santé des écosystèmes - ECSECO [ECI]
Drouaud, Jan [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Bahrman, Nasser [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Rau, Andrea [Auteur]
Génétique Animale et Biologie Intégrative [GABI]
Lejeune-Hénaut, Isabelle [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Delbreil, Bruno [Auteur]

BioEcoAgro - Equipe 1 - Functioning and adaptation of the plant in interaction with its environment
Legrand, Sylvain [Auteur correspondant]

Université de Lille
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Journal title :
Genes
Pages :
1119
Publisher :
MDPI
Publication date :
2022-07
ISSN :
2073-4425
English keyword(s) :
RNA-seq
sRNA-seq
pea
cold stress
cold acclimation
miRNA
sRNA-seq
pea
cold stress
cold acclimation
miRNA
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
(1) Background: Cold stress affects growth and development in plants and is a major environmental factor that decreases productivity. Over the past two decades, the advent of next generation sequencing (NGS) technologies ...
Show more >(1) Background: Cold stress affects growth and development in plants and is a major environmental factor that decreases productivity. Over the past two decades, the advent of next generation sequencing (NGS) technologies has opened new opportunities to understand the molecular bases of stress resistance by enabling the detection of weakly expressed transcripts and the identification of regulatory RNAs of gene expression, including microRNAs (miRNAs). (2) Methods: In this study, we performed time series sRNA and mRNA sequencing experiments on two pea (Pisum sativum L., Ps) lines, Champagne frost-tolerant and Térèse frost-sensitive, during a low temperature treatment versus a control condition. (3) Results: An integrative analysis led to the identification of 136 miRNAs and a regulation network composed of 39 miRNA/mRNA target pairs with discordant expression patterns. (4) Conclusions: Our findings indicate that the cold response in pea involves 11 miRNA families as well as their target genes related to antioxidative and multi-stress defense mechanisms and cell wall biosynthesis.Show less >
Show more >(1) Background: Cold stress affects growth and development in plants and is a major environmental factor that decreases productivity. Over the past two decades, the advent of next generation sequencing (NGS) technologies has opened new opportunities to understand the molecular bases of stress resistance by enabling the detection of weakly expressed transcripts and the identification of regulatory RNAs of gene expression, including microRNAs (miRNAs). (2) Methods: In this study, we performed time series sRNA and mRNA sequencing experiments on two pea (Pisum sativum L., Ps) lines, Champagne frost-tolerant and Térèse frost-sensitive, during a low temperature treatment versus a control condition. (3) Results: An integrative analysis led to the identification of 136 miRNAs and a regulation network composed of 39 miRNA/mRNA target pairs with discordant expression patterns. (4) Conclusions: Our findings indicate that the cold response in pea involves 11 miRNA families as well as their target genes related to antioxidative and multi-stress defense mechanisms and cell wall biosynthesis.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Source :
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