Title :

A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates

Author(s) :

Fowler, Philip [Auteur]
Wright, Carla [Auteur]
Spiers, Helen [Auteur]
Zhu, Tingting [Auteur]
Baeten, Elisabeth Ml [Auteur]
Hoosdally, Sarah [Auteur]
Gibertoni Cruz, Ana [Auteur]
Roohi, Aysha [Auteur]
Kouchaki, Samaneh [Auteur]
Walker, Timothy [Auteur]
Peto, Timothy Ea [Auteur]
Miller, Grant [Auteur]
Lintott, Chris [Auteur]
Clifton, David [Auteur]
Crook, Derrick [Auteur]
Walker, A. Sarah [Auteur]
Supply, Philip [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Journal title :

Elife

Publisher :

eLife Sciences Publication

Publication date :

2022-05-19

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Tuberculosis is a respiratory disease that is treatable with antibiotics. An increasing prevalence of resistance means that to ensure a good treatment outcome it is desirable to test the susceptibility of each infection ...
Show more >Tuberculosis is a respiratory disease that is treatable with antibiotics. An increasing prevalence of resistance means that to ensure a good treatment outcome it is desirable to test the susceptibility of each infection to different antibiotics. Conventionally, this is done by culturing a clinical sample and then exposing aliquots to a panel of antibiotics, each being present at a pre-determined concentration, thereby determining if the sample isresistant or susceptible to each sample. The minimum inhibitory concentration (MIC) of a drug is the lowestconcentration that inhibits growth and is a more useful quantity but requires each sample to be tested at a range ofconcentrations for each drug. Using 96-well broth micro dilution plates with each well containing a lyophilised pre-determined amount of an antibiotic is a convenient and cost-effective way to measure the MICs of several drugs at once for a clinical sample. Although accurate, this is still an expensive and slow process that requires highly-skilled and experienced laboratory scientists. Here we show that, through the BashTheBug project hosted on the Zooniverse citizen science platform, a crowd of volunteers can reproducibly and accurately determine the MICs for 13 drugs and that simply taking the median or mode of 11–17 independent classifications is sufficient. There is therefore a potential role for crowds to support (but not supplant) the role of experts in antibiotic susceptibility testing.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL