Structure-Function analysis of Lactiplantibacillus ...
Type de document :
Pré-publication ou Document de travail
URL permanente :
Titre :
Structure-Function analysis of Lactiplantibacillus plantarum DltE reveals D-alanylated lipoteichoic acids as direct symbiotic cues supporting Drosophila juvenile growth
Auteur(s) :
Nikolopoulos, Nikos [Auteur]
Matos, Renata [Auteur]
Institut de Génomique Fonctionnelle de Lyon [IGFL]
Ravaud, Stephanie [Auteur]
Courtin, Pascal [Auteur]
Akherraz, Houssam [Auteur]
Palussiere, Simon [Auteur]
Gueguen-Chaignon, Virginie [Auteur]
Salomon-Mallet, Marie [Auteur]
Guillot, Alain [Auteur]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
307656|||Gifu University
Chapot-Chartier, Marie-Pierre [Auteur]
Grangeasse, Christophe [Auteur]
Leulier, Francois [Auteur]
Matos, Renata [Auteur]
Institut de Génomique Fonctionnelle de Lyon [IGFL]
Ravaud, Stephanie [Auteur]
Courtin, Pascal [Auteur]
Akherraz, Houssam [Auteur]
Palussiere, Simon [Auteur]
Gueguen-Chaignon, Virginie [Auteur]
Salomon-Mallet, Marie [Auteur]
Guillot, Alain [Auteur]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
307656|||Gifu University
Chapot-Chartier, Marie-Pierre [Auteur]
Grangeasse, Christophe [Auteur]
Leulier, Francois [Auteur]
Date de production :
2022-09-14
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial ...
Lire la suite >Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by the dlt operon involved in Drosophila melanogaster's juvenile growth promotion by Lactiplantibacillus plantarum. Here, using crystallography combined with biochemical and cellular approaches, we investigate the physiological role of an uncharacterized protein (DltE) encoded by this operon. We show that LTAs but not WTAs are D-alanylated in Lactiplantibacillus plantarum NC8 cell envelope and demonstrate that DltE is a D-Ala carboxyesterase removing D-Ala from LTA. Using the mutualistic association of L. plantarum NC8 and Drosophila melanogaster as a symbiosis model, we establish that D-Ala-LTAs are direct symbiotic cues supporting intestinal peptidase expression and juvenile growth in Drosophila. Our results pave the way to probing the contribution of D-Ala-LTA to host physiology in other symbiotic models.Lire moins >
Lire la suite >Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by the dlt operon involved in Drosophila melanogaster's juvenile growth promotion by Lactiplantibacillus plantarum. Here, using crystallography combined with biochemical and cellular approaches, we investigate the physiological role of an uncharacterized protein (DltE) encoded by this operon. We show that LTAs but not WTAs are D-alanylated in Lactiplantibacillus plantarum NC8 cell envelope and demonstrate that DltE is a D-Ala carboxyesterase removing D-Ala from LTA. Using the mutualistic association of L. plantarum NC8 and Drosophila melanogaster as a symbiosis model, we establish that D-Ala-LTAs are direct symbiotic cues supporting intestinal peptidase expression and juvenile growth in Drosophila. Our results pave the way to probing the contribution of D-Ala-LTA to host physiology in other symbiotic models.Lire moins >
Langue :
Anglais
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Chemical Glycobiology
Date de dépôt :
2022-11-16T11:10:41Z
2022-11-18T11:09:07Z
2022-11-18T11:09:07Z
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- 2022.09.14.507969v1.full.pdf
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