Title :

The diversification of the antimicrobial peptides from marine worms is driven by environmental conditions

Author(s) :

Bruno, Renato [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Boidin-Wichlacz, Céline [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Melnyk, Oleg [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Zeppilli, Daniela [Auteur]
Biologie et Ecologie des Ecosystèmes Marins Profonds [BEEP]
Landon, Céline [Auteur]
Centre de biophysique moléculaire [CBM]
Thomas, Frédéric [Auteur]
Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle [MIVEGEC]
Cambon, Marie-Anne [Auteur]
Biologie et Ecologie des Ecosystèmes Marins Profonds [BEEP]
Lafond, Mickael [Auteur]
Institut des Sciences Moléculaires de Marseille [ISM2]
Mabrouk, Kamel [Auteur]
Institut de Chimie Radicalaire [ICR]
Massol, François [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Hourdez, Stéphane [Auteur]
Laboratoire d'Ecogéochimie des environnements benthiques [LECOB]
Maresca, Marc [Auteur]
Institut des Sciences Moléculaires de Marseille [ISM2]
Jollivet, Didier [Auteur]
Adaptation et diversité en milieu marin [ADMM]
Tasiemski, Aurélie [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Journal title :

Science of The Total Environment

Pages :

162875

Publisher :

Elsevier

Publication date :

2023

ISSN :

0048-9697

HAL domain(s) :

Sciences du Vivant [q-bio]/Immunologie/Immunité innée
Sciences du Vivant [q-bio]/Biologie animale/Zoologie des invertébrés

English abstract : [en]

Antimicrobial peptides (AMPs) play a key role in the external immunity of animals, offering an interesting model for studying the influence of the environment on the diversification and evolution of immune effectors., ...
Show more >Antimicrobial peptides (AMPs) play a key role in the external immunity of animals, offering an interesting model for studying the influence of the environment on the diversification and evolution of immune effectors., alvinellacin (ALV), arenicin (ARE) and polaricin (POL, a novel AMP identified here), characterized from three marine worms inhabiting contrasted habitats (‘hot’ vents, temperate and polar respectively), possess a well conserved BRICHOS domain in their precursor molecule despite a profound amino acid and structural diversification of the C-terminal part containing the core peptide. Data not only showed that ARE, ALV and POL display an optimal bactericidal activity against the bacteria typical of the habitat where each worm species lives but also that this killing efficacy is optimal under the thermochemical conditions encountered by their producers in their environment. Moreover, the correlation between species habitat and the cysteine contents of POL, ARE and ALV led us to investigate the importance of disulfide bridges in their biological efficacy as a function of abiotic pressures (pH and temperature). The construction of variants using non-proteinogenic residues instead of cysteines (α-aminobutyric acid variants) leading to AMPs devoid of disulfide bridges, provided evidence that the disulfide pattern of the three AMPs allows for a better bactericidal activity and suggests an adaptive way to sustain the fluctuations of the worm's environment. This work shows that the external immune effectors exemplified here by BRICHOS AMPs are evolving under strong diversifying environmental pressures to be structurally shaped and more efficient/specific under the ecological niche of their producer.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL