Title :

Target enrichment of long open reading frames and ultraconserved elements to link microevolution and macroevolution in non‐model organisms

Author(s) :

Ortiz‐Sepulveda, Claudia [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Genete, Mathieu [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Blassiau, Christelle [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Godé, Cécile [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Albrecht, Christian [Auteur]
Mbarara University of Science and Technology [Mbarara] [MUST]
Department of Animal Ecology and Systematics
Vekemans, Xavier [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Van Bocxlaer, Bert [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]

Journal title :

Molecular ecology resources

Pages :

659 - 679

Publisher :

Wiley/Blackwell

Publication date :

2022

ISSN :

1755-098X

English keyword(s) :

African freshwater molluscs
gene capture
genome skimming
phylogenetics
population genetics
transcriptomics (RNA-seq)

HAL domain(s) :

Sciences du Vivant [q-bio]/Génétique/Génétique animale
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
Sciences du Vivant [q-bio]/Biodiversité/Evolution [q-bio.PE]
Sciences du Vivant [q-bio]/Génétique/Génétique des populations [q-bio.PE]

English abstract : [en]

Despite the increasing accessibility of high-throughput sequencing, obtaining highquality genomic data on non-model organisms without proximate well-assembled and annotated genomes remains challenging. Here, we describe a ...
Show more >Despite the increasing accessibility of high-throughput sequencing, obtaining highquality genomic data on non-model organisms without proximate well-assembled and annotated genomes remains challenging. Here, we describe a workflow that takes advantage of distant genomic resources and ingroup transcriptomes to select and jointly enrich long open reading frames (ORFs) and ultraconserved elements (UCEs) from genomic samples for integrative studies of microevolutionary and macroevolutionary dynamics. This workflow is applied to samples of the African unionid bivalve tribe Coelaturini (Parreysiinae) at basin and continent-wide scales. Our results indicate that ORFs are efficiently captured without prior identification of intron-exon boundaries.Show less >

Language :

Anglais

Popular science :

Non

ANR Project :

Lier les processus micro- et macro-évolutifs impliqués dans les mollusques dulcicoles du Rift Est Africain

Collections :

• Évolution, Écologie et Paléontologie (Evo Eco Paleo) - UMR 8198

Source :

Harvested from HAL