Investigating Fibroblast-Induced Collagen ...
Document type :
Article dans une revue scientifique: Article original
DOI :
PMID :
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Title :
Investigating Fibroblast-Induced Collagen Gel Contraction Using a Dynamic Microscale Platform
Author(s) :
Zhang, Tianzi [Auteur]
University of Washington [Seattle]
Day, John H [Auteur]
University of Washington [Seattle]
Su, Xiaojing [Auteur]
University of Washington [Seattle]
Guadarrama, Arthur G [Auteur]
University of Wisconsin School of Medicine and Public Health
Sandbo, Nathan K [Auteur]
University of Wisconsin School of Medicine and Public Health
Esnault, Stéphane [Auteur]
University of Wisconsin School of Medicine and Public Health
Denlinger, Loren C [Auteur]
University of Wisconsin School of Medicine and Public Health
Berthier, Erwin [Auteur]
University of Washington [Seattle]
Theberge, Ashleigh B [Auteur]
University of Washington [Seattle]
University of Washington [Seattle]
Day, John H [Auteur]
University of Washington [Seattle]
Su, Xiaojing [Auteur]
University of Washington [Seattle]
Guadarrama, Arthur G [Auteur]
University of Wisconsin School of Medicine and Public Health
Sandbo, Nathan K [Auteur]
University of Wisconsin School of Medicine and Public Health
Esnault, Stéphane [Auteur]
University of Wisconsin School of Medicine and Public Health
Denlinger, Loren C [Auteur]
University of Wisconsin School of Medicine and Public Health
Berthier, Erwin [Auteur]
University of Washington [Seattle]
Theberge, Ashleigh B [Auteur]
University of Washington [Seattle]
Journal title :
Frontiers in Bioengineering and Biotechnology
Abbreviated title :
Front Bioeng Biotechnol
Volume number :
7
Pages :
196
Publication date :
2019-08-14
ISSN :
2296-4185
English keyword(s) :
coculture
collagen gel contraction
dynamic
fibrosis
mechanobiology
microfluidics
paracrine signaling
collagen gel contraction
dynamic
fibrosis
mechanobiology
microfluidics
paracrine signaling
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Mechanical forces have long been recognized as fundamental drivers in biological processes, such as embryogenesis, tissue formation and disease regulation. The collagen gel contraction (CGC) assay has served as a classic ...
Show more >Mechanical forces have long been recognized as fundamental drivers in biological processes, such as embryogenesis, tissue formation and disease regulation. The collagen gel contraction (CGC) assay has served as a classic tool in the field of mechanobiology to study cell-induced contraction of extracellular matrix (ECM), which plays an important role in inflammation and wound healing. In a conventional CGC assay, cell-laden collagen is loaded into a cell culture vessel (typically a well plate) and forms a disk-shaped gel adhering to the bottom of the vessel. The decrement in diameter or surface area of the gel is used as a parameter to quantify the degree of cell contractility. In this study, we developed a microscale CGC assay with an engineered well plate insert that uses surface tension forces to load and manipulate small volumes (14 μL) of cell-laden collagen. The system is easily operated with two pipetting steps and the microscale device moves dynamically as a result of cellular forces. We used a straightforward one-dimensional measurement as the gel contraction readout. We adapted a conventional lung fibroblast CGC assay to demonstrate the functionality of the device, observing significantly more gel contraction when human lung fibroblasts were cultured in serum-containing media vs. serum-free media ( ≤ 0.05). We further cocultured eosinophils and fibroblasts in the system, two important cellular components that lead to fibrosis in asthma, and observed that soluble factors from eosinophils significantly increase fibroblast-mediated gel contraction ( ≤ 0.01). Our microscale CGC device provides a new method for studying downstream ECM effects of intercellular cross talk using 7- to 35-fold less cell-laden gel than traditional CGC assays.Show less >
Show more >Mechanical forces have long been recognized as fundamental drivers in biological processes, such as embryogenesis, tissue formation and disease regulation. The collagen gel contraction (CGC) assay has served as a classic tool in the field of mechanobiology to study cell-induced contraction of extracellular matrix (ECM), which plays an important role in inflammation and wound healing. In a conventional CGC assay, cell-laden collagen is loaded into a cell culture vessel (typically a well plate) and forms a disk-shaped gel adhering to the bottom of the vessel. The decrement in diameter or surface area of the gel is used as a parameter to quantify the degree of cell contractility. In this study, we developed a microscale CGC assay with an engineered well plate insert that uses surface tension forces to load and manipulate small volumes (14 μL) of cell-laden collagen. The system is easily operated with two pipetting steps and the microscale device moves dynamically as a result of cellular forces. We used a straightforward one-dimensional measurement as the gel contraction readout. We adapted a conventional lung fibroblast CGC assay to demonstrate the functionality of the device, observing significantly more gel contraction when human lung fibroblasts were cultured in serum-containing media vs. serum-free media ( ≤ 0.05). We further cocultured eosinophils and fibroblasts in the system, two important cellular components that lead to fibrosis in asthma, and observed that soluble factors from eosinophils significantly increase fibroblast-mediated gel contraction ( ≤ 0.01). Our microscale CGC device provides a new method for studying downstream ECM effects of intercellular cross talk using 7- to 35-fold less cell-laden gel than traditional CGC assays.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Collections :
Submission date :
2023-10-23T10:02:35Z
2024-03-16T08:57:28Z
2024-03-16T08:57:28Z
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