Analytical and computational workflow for ...
Document type :
Article dans une revue scientifique: Article original
PMID :
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Title :
Analytical and computational workflow for in-depth analysis of oxidized complex lipids in blood plasma.
Author(s) :
Criscuolo, A. [Auteur]
Nepachalovich, P. [Auteur]
Garcia Del Rio, Diego Fernando [Auteur]
Lange, M. [Auteur]
Ni, Z. [Auteur]
Baroni, M. [Auteur]
Cruciani, G. [Auteur]
Goracci, L. [Auteur]
Blüher, M. [Auteur]
Fedorova, M. [Auteur]
Nepachalovich, P. [Auteur]
Garcia Del Rio, Diego Fernando [Auteur]
Lange, M. [Auteur]
Ni, Z. [Auteur]
Baroni, M. [Auteur]
Cruciani, G. [Auteur]
Goracci, L. [Auteur]
Blüher, M. [Auteur]
Fedorova, M. [Auteur]
Journal title :
Nature Communications
Abbreviated title :
Nat Commun
Volume number :
13
Pages :
6547
Publication date :
2022-11-06
ISSN :
2041-1723
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Lipids are a structurally diverse class of biomolecules which can undergo a variety of chemical modifications. Among them, lipid (per)oxidation attracts most of the attention due to its significance in the regulation of ...
Show more >Lipids are a structurally diverse class of biomolecules which can undergo a variety of chemical modifications. Among them, lipid (per)oxidation attracts most of the attention due to its significance in the regulation of inflammation, cell proliferation and death programs. Despite their apparent regulatory significance, the molecular repertoire of oxidized lipids remains largely elusive as accurate annotation of lipid modifications is complicated by their low abundance and often unknown, biological context-dependent structural diversity. Here, we provide a workflow based on the combination of bioinformatics and LC-MS/MS technologies to support identification and relative quantification of oxidized complex lipids in a modification type- and position-specific manner. The developed methodology is used to identify epilipidomics signatures of lean and obese individuals with and without type 2 diabetes. The characteristic signature of lipid modifications in lean individuals, dominated by the presence of modified octadecanoid acyl chains in phospho- and neutral lipids, is drastically shifted towards lipid peroxidation-driven accumulation of oxidized eicosanoids, suggesting significant alteration of endocrine signalling by oxidized lipids in metabolic disorders.Show less >
Show more >Lipids are a structurally diverse class of biomolecules which can undergo a variety of chemical modifications. Among them, lipid (per)oxidation attracts most of the attention due to its significance in the regulation of inflammation, cell proliferation and death programs. Despite their apparent regulatory significance, the molecular repertoire of oxidized lipids remains largely elusive as accurate annotation of lipid modifications is complicated by their low abundance and often unknown, biological context-dependent structural diversity. Here, we provide a workflow based on the combination of bioinformatics and LC-MS/MS technologies to support identification and relative quantification of oxidized complex lipids in a modification type- and position-specific manner. The developed methodology is used to identify epilipidomics signatures of lean and obese individuals with and without type 2 diabetes. The characteristic signature of lipid modifications in lean individuals, dominated by the presence of modified octadecanoid acyl chains in phospho- and neutral lipids, is drastically shifted towards lipid peroxidation-driven accumulation of oxidized eicosanoids, suggesting significant alteration of endocrine signalling by oxidized lipids in metabolic disorders.Show less >
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Submission date :
2023-12-13T04:03:23Z
2024-01-22T11:46:56Z
2024-01-22T11:46:56Z
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