Title :

Multivariate analysis of chemical and genetic diversity of wild Humulus lupulus L. (hop) collected in situ in northern France

Author(s) :

Paguet, Anne-Sophie [Auteur]
Siah, Ali [Auteur]
BioEcoAgro - Equipe 5 - Specialized Metabolites of Plant Origin
Lefèvre, Gabriel [Auteur]
Moureu, Sophie [Auteur]
JUNIA [JUNIA]
Université de Liège
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Cadalen, Thierry [Auteur]
SFR Condorcet
Institut Charles Viollette (ICV) - ULR 7394 [ICV]
Samaillie, Jennifer [Auteur]
BioEcoAgro - Equipe 5 - Specialized Metabolites of Plant Origin
JUNIA [JUNIA]
Université de Liège
Michels, Franck [Auteur]
Deracinois, Barbara [Auteur]
BioEcoAgro - Equipe 7 - Health benefit of protein hydrolysates and agro-food co-products: control of production, characterization and valorisation
Flahaut, Christophe [Auteur]
BioEcoAgro - Equipe 7 - Health benefit of protein hydrolysates and agro-food co-products: control of production, characterization and valorisation
Alves Dos Santos, Harmony [Auteur]
Etienne-Debaecker, Audrey [Auteur]
Rambaud, Caroline [Auteur]
BioEcoAgro - Equipe 5 - Specialized Metabolites of Plant Origin
Chollet, Sylvie [Auteur]
Université de Liège
Université d'Artois [UA]
Molinié, Roland [Auteur]
BioEcoAgro - Equipe 5 - Specialized Metabolites of Plant Origin
BioEcoAgro BIOPI-UPJV
Fontaine, Jean-Xavier [Auteur]
BioEcoAgro - Equipe 5 - Specialized Metabolites of Plant Origin
BioEcoAgro BIOPI-UPJV
Waterlot, Christophe [Auteur]
Laboratoire de Génie Civil et Géo-Environnement (LGCgE) - ULR 4515 [LGCgE]
JUNIA [JUNIA]
Fauconnier, Marie-Laure [Auteur]
Gembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
Sahpaz, Sevser [Auteur]
BioEcoAgro - Equipe 5 - Specialized Metabolites of Plant Origin
JUNIA [JUNIA]
Université de Liège
Rivière, Céline [Auteur]
BioEcoAgro - Equipe 5 - Specialized Metabolites of Plant Origin
Faculté de Médecine Henri Warembourg - Université de Lille

Journal title :

Phytochemistry

Pages :

113508

Publication date :

2023-01

ISSN :

0031-9422

English keyword(s) :

Cannabaceae
Wild hops
SSR finger
printing
Soil analysis
HS-SPME GC-MS
UHPLC-UV-MS
Untargeted metabolomics
Multiple factor analysis
Humulus lupulus L

HAL domain(s) :

Sciences du Vivant [q-bio]/Biologie végétale

English abstract : [en]

The hop plant (Humulus lupulus L.) has been exploited for a long time for both its brewing and medicinal uses, due in particular to its specific chemical composition. These last years, hop cultivation that was in decline ...
Show more >The hop plant (Humulus lupulus L.) has been exploited for a long time for both its brewing and medicinal uses, due in particular to its specific chemical composition. These last years, hop cultivation that was in decline has been experiencing a renewal for several reasons, such as a craze for strongly hopped aromatic beers. In this context, the present work aims at investigating the genetic and chemical diversity of fifty wild hops collected from different locations in Northern France. These wild hops were compared to ten commercial varieties and three heirloom varieties cultivated in the same sampled geographical area. Genetic analysis relying on genome fingerprinting using 11 microsatellite markers showed a high level of diversity. A total of 56 alleles were determined with an average of 10.9 alleles per locus and assessed a significant population structure (mean pairwise FST = 0.29). Phytochemical characterization of hops was based on volatile compound analysis by HS-SPME GC-MS, quantification of the main prenylated phenolic compounds by UHPLC-UV as well as untargeted metabolomics by UHPLC-HRMS and revealed a high level of chemical diversity among the assessed wild ac-cessions. In particular, analysis of volatile compounds revealed the presence of some minor but original com-pounds, such as aromadendrene, allo-aromadendrene, isoledene, fl-guaiene, alpha-ylangene and fl-pinene in some wild accessions; while analysis of phenolic compounds showed high content of fl-acids in these wild accessions, up to 2.37% of colupulone. Genetic diversity of wild hops previously observed was hence supported by their chemical diversity. Sample soil analysis was also performed to get a pedological classification of these different collection sites. Results of the multivariate statistical analysis suggest that wild hops constitute a huge pool of chemical and genetic diversity of this species.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Laboratoire Génie Civil et géo-Environnement (LGCgE) - ULR 4515

Source :

Harvested from HAL