Titre :

Deciphering the transcriptional landscape of human pluripotent stem cell-derived GnRH neurons: the role of Wnt signaling in patterning the neural fate.

Auteur(s) :

Wang, Y. [Auteur]
Madhusudan, S. [Auteur]
Cotellessa, Ludovica [Auteur]
Lille Neurosciences & Cognition - U 1172 [LilNCog]
Kvist, J. [Auteur]
Eskici, N. [Auteur]
Yellapragada, V. [Auteur]
Pulli, K. [Auteur]
Lund, C. [Auteur]
Vaaralahti, K. [Auteur]
Tuuri, T. [Auteur]
Giacobini, Paolo [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172
Raivio, Taneli [Auteur]
Helsingin yliopisto = Helsingfors universitet = University of Helsinki

Titre de la revue :

Stem Cells

Numéro :

40

Pagination :

1107–1121

Éditeur :

Oxford University Press

Date de publication :

2022-09-29

ISSN :

1549-4918

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Hypothalamic gonadotropin-releasing hormone (GnRH) neurons lay the foundation for human development and reproduction; however, the critical cell populations and the entangled mechanisms underlying the development of human ...
Lire la suite >Hypothalamic gonadotropin-releasing hormone (GnRH) neurons lay the foundation for human development and reproduction; however, the critical cell populations and the entangled mechanisms underlying the development of human GnRH neurons remain poorly understood. Here, by using our established human pluripotent stem cell-derived GnRH neuron model, we decoded the cellular heterogeneity and differentiation trajectories at the single-cell level. We found that a glutamatergic neuron population, which generated together with GnRH neurons, showed similar transcriptomic properties with olfactory sensory neuron and provided the migratory path for GnRH neurons. Through trajectory analysis, we identified a specific gene module activated along the GnRH neuron differentiation lineage, and we examined one of the transcription factors, DLX5, expression in human fetal GnRH neurons. Furthermore, we found that Wnt inhibition could increase DLX5 expression and improve the GnRH neuron differentiation efficiency through promoting neurogenesis and switching the differentiation fates of neural progenitors into glutamatergic neurons/GnRH neurons. Our research comprehensively reveals the dynamic cell population transition and gene regulatory network during GnRH neuron differentiation.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Lille Neurosciences & Cognition (LilNCog) - U 1172

Date de dépôt :

2024-01-16T00:39:56Z
2024-09-11T08:13:02Z