Title :

Comparing flow cytometry and microscopy in the quantification of vital aquatic organisms in ballast water

Author(s) :

Peperzak, Louis [Auteur]
Royal Netherlands Institute for Sea Research [NIOZ]
Zetsche, Eva-Maria [Auteur]
Royal Netherlands Institute for Sea Research [NIOZ]
Gollasch, Stephan [Auteur]
Royal Netherlands Institute for Sea Research [NIOZ]
Artigas, Luis Felipe [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Bonato, Simon [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Université du Littoral Côte d'Opale [ULCO]
Creach, Veronique [Auteur]
Centre for Environment, Fisheries and Aquaculture Science [Lowestoft] [CEFAS]
de Vré, Pieter [Auteur]
Dubelaar, George B.J. [Auteur]
Henneghien, Joël [Auteur]
Hess-Erga, Ole-Kristian [Auteur]
Langelaar, Roland [Auteur]
Larsen, Aud [Auteur]
Norwegian Research Center [NORCE]
Maurer, Brian [Auteur]
Mosselaar, Albert [Auteur]
Reavie, Euan [Auteur]
Rijkeboer, Machteld [Auteur]
Tobiesen, August [Auteur]

Journal title :

Journal of Marine Engineering and Technology

Pages :

68-77

Publisher :

Taylor & Francis

Publication date :

2020

ISSN :

2046-4177

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The ability to quantify vital aquatic organisms in the 2–50 µm size range was compared between five different flow cytometers and several different microscopes. Counts of calibration beads, algal monocultures of different ...
Show more >The ability to quantify vital aquatic organisms in the 2–50 µm size range was compared between five different flow cytometers and several different microscopes. Counts of calibration beads, algal monocultures of different sizes as well as organisms in a Wadden Sea sample were compared. Flow cytometers and microscopes delivered different bead concentrations. These differences between the instruments became larger for algal monocultures and were even higher for the Wadden Sea sample. It was observed that the concentration differences were significant between flow cytometer and microscope counts, and that this difference increased with the size of the objects counted. Microscope counts were more accurate for larger (50 µm) objects because cytometers struggled with bigger particles that clogged the instruments. Contrary to microscopy, the flow cytometers were capable of accurately enumerating cultured cells in the 2–10 µm size range and cells in the lower size range of the 10–50 µm size class. Flow cytometers were also well-suited to assess low abundance samples due to their ability to process larger volumes than microscopes. The results were used to indicate which tools are suitable for ballast water monitoring: flow cytometry is a suitable technology for an indicative and real time analysis of ballast water samples whilst only microscopy would be robust enough for detailed taxonomical analyses.Show less >

Language :

Anglais

Popular science :

Non

Collections :

• Laboratoire d'Océanologie et de Géosciences (LOG) - UMR 8187

Source :

Harvested from HAL