Title :

Cloning and Molecular Characterization of the Recombinant CVB4E2 Immunogenic Viral Protein (rVP1), as a Potential Subunit Protein for Vaccine and Immunodiagnostic Reagent Candidate

Author(s) :

Hassine, Ikbel Hadj [Auteur]
Université de Monastir - University of Monastir [UM]
Gharbi, Jawhar [Auteur]
King Faisal University [KFU]
Amara, Imene [Auteur]
Alyami, Ameera [Auteur]
Subei, Reem [Auteur]
Almalki, Mohammed [Auteur]
King Faisal University [KFU]
Hober, Didier [Auteur]
Laboratoire de virologie - ULR 3610
M'hadheb, Manel Ben [Auteur]
Université de Monastir - University of Monastir [UM]

Journal title :

Microorganisms

Abbreviated title :

Microorganisms

Volume number :

11

Pages :

1192

Publication date :

2023-06-13

ISSN :

2076-2607

English keyword(s) :

bioinformatics
molecular characterization
recombinant rVP1
coxsackievirus B4

HAL domain(s) :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
Sciences du Vivant [q-bio]

English abstract : [en]

The aim of the present study was, first, to clone the VP1 gene of the human coxsackievirus B4 strain E2 (CVB4E2) in the prokaryotic pUC19 plasmid expression vector then to compare it with the structural capsid proteins of ...
Show more >The aim of the present study was, first, to clone the VP1 gene of the human coxsackievirus B4 strain E2 (CVB4E2) in the prokaryotic pUC19 plasmid expression vector then to compare it with the structural capsid proteins of the same strain using bioinformatic tools. PCR colony amplification followed through a restriction digestion analysis and sequencing process which affirmed the success of the cloning process. SDS-PAGE and Western Blotting were used to characterize the purified recombinant viral protein expressed in bacteria cells. The BLASTN tool revealed that the nucleotide sequence of the recombinant VP1 (rVP1) expressed by pUC19 highly matched the target nucleotide sequence of the diabetogenic CVB4E2 strain. Secondary structure and three-dimension structure prediction suggested that rVP1, such as wild-type VP1, is chiefly composed of random coils and a high percentage of exposed amino acids. Linear B-cell epitope prediction showed that several antigenic epitopes are likely present in rVP1 and CVB4E2 VP1 capsid protein. Additionally, phosphorylation site prediction revealed that both proteins may affect the signal transduction of host cells and can be involved in virus virulence. The present work highlights the usefulness of cloning and bioinformatics characterizations for gene investigation. Furthermore, the collected data are helpful for future experimental research related to the development of immunodiagnostic reagents and subunit vaccines based on the expression of immunogenic viral capsid proteins.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CHU Lille

Collections :

• Laboratoire de virologie - ULR 3610

Submission date :

2024-01-17T22:02:33Z
2024-02-12T15:23:32Z