Title :

Coxsackievirus-B4 Infection of Human Primary Pancreatic Ductal Cell Cultures Results in Impairment of Differentiation into Insulin-Producing Cells.

Author(s) :

Bertin, Antoine [Auteur]
Laboratoire de virologie - ULR 3610
Sane, Famara [Auteur]
Laboratoire de virologie - ULR 3610
Gmyr, Valery [Auteur]
Recherche translationnelle sur le diabète - U 1190 [RTD]
Lobert, Delphine [Auteur]
Laboratoire de virologie - ULR 3610
Dechaumes, Arthur [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Pattou KERR-CONTE, Julie [Auteur]
Recherche translationnelle sur le diabète (RTD) - U1190
Pattou, Francois [Auteur]
Recherche translationnelle sur le diabète (RTD) - U1190
Hober, Didier [Auteur]
Laboratoire de virologie - ULR 3610

Journal title :

Viruses

Abbreviated title :

Viruses

Volume number :

11

Pages :

597

Publication date :

2019-07-06

ISSN :

1999-4915

English keyword(s) :

enterovirus
in vitro
c-peptide
insulin mRNA
RT-PCR

HAL domain(s) :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie

English abstract : [en]

Coxsackievirus-B4 (CV-B4) E2 can persist in the pancreatic ductal-like cells (Panc-1 cell line), which results in an impaired differentiation of these cells into islet-like cell aggregates (ICA). In this study, primary ...
Show more >Coxsackievirus-B4 (CV-B4) E2 can persist in the pancreatic ductal-like cells (Panc-1 cell line), which results in an impaired differentiation of these cells into islet-like cell aggregates (ICA). In this study, primary pancreatic ductal cells obtained as a by-product of islet isolation from the pancreas of seven brain-dead adults were inoculated with CV-B4 E2, followed-up for 29 days, and the impact was investigated. Viral titers in culture supernatants were analyzed throughout the culture. Intracellular viral RNA was detected by RT-PCR. Levels of ductal cell marker CK19 mRNA and of insulin mRNA were evaluated by qRT-PCR. The concentration of c-peptide in supernatants was determined by ELISA. Ductal cells exposed to trypsin and serum-free medium formed ICA and resulted in an increased insulin secretion. Ductal cells from five brain-dead donors were severely damaged by CV-B4 E2, whereas the virus persisted in cultures of cells obtained from the other two. The ICAs whose formation was induced on day 14 post-inoculation were scarce and appeared tiny in infected cultures. Also, insulin mRNA expression and c-peptide levels were strongly reduced compared to the controls. In conclusion, CV-B4 E2 lysed human primary pancreatic ductal cells or persisted in these cells, which resulted in the impairment of differentiation into insulin-producing cells.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CHU Lille

Collections :

• Laboratoire de virologie - ULR 3610
• Recherche translationnelle sur le diabète (RTD) - U1190

Submission date :

2024-01-17T22:31:44Z
2024-02-13T10:30:24Z