Titre :

Evaluation of greenness and analytical performances of separative methods for chiral separation of novel lactam‐based P2RX7‐antagonists

Auteur(s) :

Avigo, Lorenzo [Auteur]
Furman, Christophe [Auteur]
Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 [RID-AGE]
Ghinet, Alina [Auteur]
Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 [RID-AGE]
JUNIA [JUNIA]
Sandu, Teodora [Auteur]
JUNIA [JUNIA]
Wynendaele, Evelien [Auteur]
Wielgomas, Bartosz [Auteur]
de Spiegeleer, Bart [Auteur]
Lipka, Emmanuelle [Auteur]

Titre de la revue :

ELECTROPHORESIS

Éditeur :

Wiley-VCH Verlag

Date de publication :

2023-10-04

ISSN :

0173-0835

Discipline(s) HAL :

Chimie

Résumé en anglais : [en]

Abstract In this work, a preparative supercritical fluid chromatography (SFC) method was first developed to separate a series of chiral compounds evaluated as lactam‐based P2RX7 antagonists. Subsequently, high‐performance ...
Lire la suite >Abstract In this work, a preparative supercritical fluid chromatography (SFC) method was first developed to separate a series of chiral compounds evaluated as lactam‐based P2RX7 antagonists. Subsequently, high‐performance liquid chromatography, SFC, and capillary electrophoresis (CE) were comparatively investigated as QC tools to determine the enantiomeric purity of the separated isomers, including analytical performance and greenness. The screening of the best conditions was carried out in liquid and SFC on the nine derivatives and the amylose tris (3,5‐dimethylphenylcarbamate)‐based chiral stationary phase was found to be highly efficient. The same screening was carried out in CE and very different conditions, either in acidic or basic background electrolyte and different cyclodextrins used as chiral selectors, allowed the separation of six of the nine derivatives. 1‐((3,4‐Dichlorophenyl)carbamoyl)‐5‐oxopyrrolidine‐2‐carboxylic acid (compound 1 ) was chosen as a probe, and its semi‐preparative separation by SFC and enantiomeric verification using the three techniques are presented. Its limit of detection and limit of quantification are calculated for each method. Finally, the greenness of each quality control method was evaluated.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement (RID-AGE) - U1167

Source :

Harvested from HAL