Title :

Immunostimulatory Effect of Flagellin on MDR-Klebsiella-Infected Human Airway Epithelial Cells

Author(s) :

Van Linge, Christine [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
Hulme, Katina [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
Peters-Sengers, Hessel [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
Sirard, Jean-Claude [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Goessens, Wil [Auteur]
Erasmus University Medical Center [Rotterdam] [Erasmus MC]
de Jong, Menno [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
Russell, Colin [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
Boston University [Boston] [BU]
de Vos, Alex [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
van der Poll, Tom [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]

Journal title :

International Journal of Molecular Sciences

Pages :

309

Publisher :

MDPI

Publication date :

2023-12-25

ISSN :

1661-6596

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Pneumonia caused by multi-drug-resistant Klebsiella pneumoniae (MDR-Kpneu) poses a major public health threat, especially to immunocompromised or hospitalized patients. This study aimed to determine the immunostimulatory ...
Show more >Pneumonia caused by multi-drug-resistant Klebsiella pneumoniae (MDR-Kpneu) poses a major public health threat, especially to immunocompromised or hospitalized patients. This study aimed to determine the immunostimulatory effect of the Toll-like receptor 5 ligand flagellin on primary human lung epithelial cells during infection with MDR-Kpneu. Human bronchial epithelial (HBE) cells, grown on an air–liquid interface, were inoculated with MDR-Kpneu on the apical side and treated during ongoing infection with antibiotics (meropenem) and/or flagellin on the basolateral and apical side, respectively; the antimicrobial and inflammatory effects of flagellin were determined in the presence or absence of meropenem. In the absence of meropenem, flagellin treatment of MDR-Kpneu-infected HBE cells increased the expression of antibacterial defense genes and the secretion of chemokines; moreover, supernatants of flagellin-exposed HBE cells activated blood neutrophils and monocytes. However, in the presence of meropenem, flagellin did not augment these responses compared to meropenem alone. Flagellin did not impact the outgrowth of MDR-Kpneu. Flagellin enhances antimicrobial gene expression and chemokine release by the MDR-Kpneu-infected primary human bronchial epithelium, which is associated with the release of mediators that activate neutrophils and monocytes. Topical flagellin therapy may have potential to boost immune responses in the lung during pneumonia.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL