Title :

Intrinsic Flexibility beyond the Highly Ordered DNA Tetrahedron: An Integrative Spectroscopic and Molecular Dynamics Approach

Author(s) :

Zerbetto, Mirco [Auteur]
Università degli Studi di Padova = University of Padua [Unipd]
Saint-Pierre, Christine [Auteur]
SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé [SYMMES]
Piserchia, Andrea [Auteur]
Università degli Studi di Padova = University of Padua [Unipd]
Torrengo, Simona [Auteur]
SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé [SYMMES]
Gambarelli, Serge [Auteur]
SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé [SYMMES]
Abergel, Daniel [Auteur]
Laboratoire des biomolécules [LBM UMR 7203]
Polimeno, Antonino [Auteur]
Dipartimento di Scienze Chimiche [Padova]
Gasparutto, Didier [Auteur]
SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé [SYMMES]
Sicoli, Giuseppe [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516

Journal title :

Journal of Physical Chemistry Letters

Volume number :

14

Pages :

10032-10038

Publication date :

2023

English keyword(s) :

Chemical structure
Electron paramagnetic resonance spectroscopy
Genetics
Probes
Quantum mechanics

HAL domain(s) :

Chimie

English abstract : [en]

Since the introduction of DNA-based architectures, in the past decade, DNA tetrahedrons have aroused great interest. Applications of such nanostructures require structural control, especially in the perspective of their ...
Show more >Since the introduction of DNA-based architectures, in the past decade, DNA tetrahedrons have aroused great interest. Applications of such nanostructures require structural control, especially in the perspective of their possible functionalities. In this work, an integrated approach for structural characterization of a tetrahedron structure is proposed with a focus on the fundamental biophysical aspects driving the assembly process. To address such an issue, spin-labeled DNA sequences are chemically synthesized, self-assembled, and then analyzed by Continuous-Wave (CW) and pulsed Electron Paramagnetic Resonance (EPR) spectroscopy. Interspin distance measurements based on PELDOR/DEER techniques combined with molecular dynamics (MD) thus revealed unexpected dynamic heterogeneity and flexibility of the assembled structures. The observation of flexibility in these ordered 3D structures demonstrates the sensitivity of this approach and its effectiveness in accessing the main dynamic and structural features with unprecedented resolution.Show less >

Language :

Anglais

Audience :

Non spécifiée

Popular science :

Non

Administrative institution(s) :

ENSCL
CNRS
Université de Lille

Collections :

• Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516

Research team(s) :

Propriétés magnéto structurales des matériaux (PMSM)

Submission date :

2024-02-21T17:11:55Z
2024-02-27T10:30:58Z