Title :

Bone Samples Extracted from Embalmed Subjects Are Not Appropriate for the Assessment of Bone Quality at the Molecular Level Using Raman Spectroscopy

Author(s) :

Pascart, Tristan [Auteur]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Hôpital Saint Philibert [Lomme]
Cortet, Bernard [Auteur]
Service de rhumatologie[Lille]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Olejnik, Cecile [Auteur]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Marrow Adiposity & Bone Lab (MABLab) - ULR 4490
Paccou, Julien [Auteur]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Service de rhumatologie[Lille]
Marrow Adiposity & Bone Lab (MABLab) - ULR 4490
Migaud, Henri [Auteur]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Marrow Adiposity & Bone Lab (MABLab) - ULR 4490
Cotten, Anne [Auteur]
Radiologie et Imagerie Musculo-Squelettique [CHU Lille]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Delannoy, Yann [Auteur]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Unité de Taphonomie médico-légale et Anatomie - ULR 7367 [UTML&A]
During, Alexandrine [Auteur]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Marrow Adiposity & Bone Lab (MABLab) - ULR 4490
Hardouin, Pierre [Auteur]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Université du Littoral Côte d'Opale [ULCO]
Penel, Guillaume [Auteur]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Falgayrac, Guillaume [Auteur]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]

Journal title :

Analytical Chemistry

Pages :

2777-2783

Publisher :

American Chemical Society

Publication date :

2016-03

ISSN :

0003-2700

HAL domain(s) :

Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Rhumatologie et système ostéo-articulaire

English abstract : [en]

Bone samples extracted from embalmed cadavers are commonly used as controls in the study of bone. The effects of embalmment on the molecular composition of bone are unknown. The objective of this study was to determine the ...
Show more >Bone samples extracted from embalmed cadavers are commonly used as controls in the study of bone. The effects of embalmment on the molecular composition of bone are unknown. The objective of this study was to determine the effect of embalmment on the molecular composition and structure of bone, as evaluated by Raman spectroscopy. Bone samples of femoral heads from five embalmed donors and five fresh-frozen donors were compared using Raman microspectroscopy with DuoScan technology. Physicochemical parameters simultaneously describing the organic and mineral phases of bone were compared using the Mann–Whitney U test. Partial least squares discriminant analysis (PLS-DA) was used to determine specific Raman spectral features of each group. Study of the mineral phase showed a 15% reduction of the mineral-to-matrix ratio (p < 0.001), an 8% decrease of type B carbonate substitution (p < 0.001), and a 2% increase in crystallinity (p < 0.001) in the embalmed donors group compared to those of the fresh donors group. Regarding the organic phase of bone, the hydroxyproline-to-proline ratio was increased by 18% in the embalmed group (p < 0.001), with no variation in both the relative proteoglycan content (GAG/CH3) (p = 0.08) and collagen maturity (p = 0.57). PLS-DA showed that the embalmed group was characterized mainly by peaks assigned to hydroxyproline, lipids, and collagen. Embalmment induces significant modifications of the molecular composition of bone. Bone samples from embalmed subjects should be avoided as controls for Raman spectroscopy studies. Preservation procedures performed prior to bone sampling should be reported in studies using human cadaver samples.Show less >

Language :

Anglais

Popular science :

Non

Collections :

• Marrow Adiposity & Bone Lab (MABLab) - ULR 4490
• Unité de Taphonomie Médico-Légale & d’Anatomie (UTMLA) - ULR 7367

Submission date :

2024-02-27T11:11:59Z