Title :

Structure-function-dynamics relationships in the peculiar Planktothrix PCC7805 OCP1: Impact of his-tagging and carotenoid type.

Author(s) :

Wilson, Adjélé [Auteur]
Institut de Biologie Intégrative de la Cellule [I2BC]
Mécanismes régulateurs chez les organismes photosynthétiques [MROP]
Andreeva, Elena [Auteur]
Groupe Dynamique et Cinétique des processus moléculaires [IBS-DYNAMOP]
Niziński, Stanisław [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Talbot, Léa [Auteur]
Institut de Biologie Intégrative de la Cellule [I2BC]
Mécanismes régulateurs chez les organismes photosynthétiques [MROP]
Hartmann, Elisabeth [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Schlichting, Ilme [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Burdzinski, Gotard [Auteur]
Sliwa, Michel [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
Kirilovsky, Diana [Auteur]
Institut de Biologie Intégrative de la Cellule [I2BC]
Mécanismes régulateurs chez les organismes photosynthétiques [MROP]
Colletier, Jacques-Philippe [Auteur]
Institut de biologie structurale [IBS - UMR 5075 ]

Journal title :

Biochim Biophys Acta Bioenerg

Abbreviated title :

Biochim Biophys Acta Bioenerg

Pages :

148584

Publication date :

2022-06-27

ISSN :

1879-2650

English keyword(s) :

Cyanobacteria
Flash photolysis
Photosynthetic pigments
Structure function relationships
X-ray diffraction

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

The orange carotenoid protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection. Here, we report on the functional, spectral and structural characteristics of the peculiar Planktothrix PCC7805 OCP ...
Show more >The orange carotenoid protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection. Here, we report on the functional, spectral and structural characteristics of the peculiar Planktothrix PCC7805 OCP (Plankto-OCP). We show that this OCP variant is characterized by higher photoactivation and recovery rates, and a stronger energy-quenching activity, compared to other OCP studied thus far. We characterize the effect of the functionalizing carotenoid and of his-tagging on these reactions, and identify the time scales on which these modifications affect photoactivation. The presence of a his-tag at the C-terminus has a large influence on photoactivation, thermal recovery and PBS-fluorescence quenching, and likewise for the nature of the carotenoid that additionally affects the yield and characteristics of excited states and the ns-s dynamics of photoactivated OCP. By solving the structures of Plankto-OCP in the ECN- and CAN-functionalized states, each in two closely-related crystal forms, we further unveil the molecular breathing motions that animate Plankto-OCP at the monomer and dimer levels. We finally discuss the structural changes that could explain the peculiar properties of Plankto-OCP.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516

Research team(s) :

Dynamics, Nanoscopy & Chemometrics (DyNaChem)

Submission date :

2024-02-28T22:24:39Z
2024-03-13T12:32:08Z