Title :

Identifying microbial species by single-molecule DNA optical mapping and resampling statistics.

Author(s) :

Bouwens, A. [Auteur]
Deen, J. [Auteur]
Vitale, Raffaele [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
D'huys, L. [Auteur]
Goyvaerts, V. [Auteur]
Descloux, A. [Auteur]
Borrenberghs, D. [Auteur]
Grussmayer, K. [Auteur]
Lukes, T. [Auteur]
Camacho, R. [Auteur]
Su, J. [Auteur]
Ruckebusch, Cyril [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
Lasser, T. [Auteur]
Van De Ville, D. [Auteur]
Hofkens, J. [Auteur]
Radenovic, A. [Auteur]
Frans Janssen, K. P. [Auteur]

Journal title :

NAR Genom Bioinform

Abbreviated title :

NAR Genom Bioinform

Volume number :

2

Pages :

lqz007

Publication date :

2021-02-22

ISSN :

2631-9268

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Single-molecule DNA mapping has the potential to serve as a powerful complement to high-throughput sequencing in metagenomic analysis. Offering longer read lengths and forgoing the need for complex library preparation and ...
Show more >Single-molecule DNA mapping has the potential to serve as a powerful complement to high-throughput sequencing in metagenomic analysis. Offering longer read lengths and forgoing the need for complex library preparation and amplification, mapping stands to provide an unbiased view into the composition of complex viromes and/or microbiomes. To fully enable mapping-based metagenomics, sensitivity and specificity of DNA map analysis and identification need to be improved. Using detailed simulations and experimental data, we first demonstrate how fluorescence imaging of surface stretched, sequence specifically labeled DNA fragments can yield highly sensitive identification of targets. Second, a new analysis technique is introduced to increase specificity of the analysis, allowing even closely related species to be resolved. Third, we show how an increase in resolution improves sensitivity. Finally, we demonstrate that these methods are capable of identifying species with long genomes such as bacteria with high sensitivity.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516

Submission date :

2024-02-28T22:45:23Z
2024-03-15T13:05:32Z