Title :

Simultaneous Quantification of Trehalose and Trehalose 6-Phosphate by Hydrophilic Interaction Chromatography/Electrospray Accurate Mass Spectrometry with Application in Non-Targeted Metabolomics

Author(s) :

Tao, Ye [Auteur]
Université de Technologie de Compiègne [UTC]
Rossez, Yannick [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Bortolus, Clovis [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Duma, Luminita [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Université de Technologie de Compiègne [UTC]
Dubar, Faustine [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Merlier, Franck [Auteur]
Université de Technologie de Compiègne [UTC]
Duma, Luminita [Auteur]

Journal title :

Molecules

Abbreviated title :

Molecules

Volume number :

28

Pages :

3443

Publisher :

MDPI AG

Publication date :

2023-04-13

ISSN :

1420-3049

English keyword(s) :

trehalose
trehalose 6-phosphate
sugar
sugar phosphate
high resolution time-of-flight MS
HILIC

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

High-resolution mass spectrometry (HRMS) was coupled with ultra-high-performance liquid chromatography (UHPLC) to simultaneously quantify trehalose and trehalose 6-phosphate without derivatization or sample preparation. ...
Show more >High-resolution mass spectrometry (HRMS) was coupled with ultra-high-performance liquid chromatography (UHPLC) to simultaneously quantify trehalose and trehalose 6-phosphate without derivatization or sample preparation. The use of full scan mode and exact mass analysis also makes it possible to carry out metabolomic analyses as well as semi-quantification. In addition, the use of different clusters in negative mode makes it possible to compensate for deficiencies in linearity and inerrant saturation at time-of-flight detectors. The method has been approved and validated for different matrices, yeasts, and bacteria, and has shown differentiation between bacteria as a function of growth temperatures.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Glycobiology in fungal Pathogenesis and Clinical Applications

Submission date :

2024-03-01T14:22:54Z
2024-03-04T08:28:52Z
2024-03-04T08:44:34Z