Title :

Luteolin Isolated from Juncus acutus L., a Potential Remedy for Human Coronavirus 229E

Author(s) :

Hakem, Asma [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Desmarets, Lowiese [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Sahli, Ramla [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Malek, Rawen Ben [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Camuzet, Charline [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
François, Nathan [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Lefèvre, Gabriel [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Samaillie, Jennifer [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Moureu, Sophie [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Sahpaz, Sevser [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Belouzard, Sandrine [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Ksouri, Riadh [Auteur]
Seron, Karin [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Rivière, Céline [Auteur correspondant]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158

Journal title :

Molecules

Pages :

4263

Publisher :

MDPI

Publication date :

2023-05-23

ISSN :

1420-3049

HAL domain(s) :

Sciences du Vivant [q-bio]
Chimie

English abstract : [en]

The COVID-19 pandemic, caused by SARS-CoV-2, addressed the lack of specific antiviral drugs against coronaviruses. In this study, bioguided fractionation performed on both ethyl acetate and aqueous sub-extracts of Juncus ...
Show more >The COVID-19 pandemic, caused by SARS-CoV-2, addressed the lack of specific antiviral drugs against coronaviruses. In this study, bioguided fractionation performed on both ethyl acetate and aqueous sub-extracts of Juncus acutus stems led to identifying luteolin as a highly active antiviral molecule against human coronavirus HCoV-229E. The apolar sub-extract (CH2Cl2) containing phenanthrene derivatives did not show antiviral activity against this coronavirus. Infection tests on Huh-7 cells, expressing or not the cellular protease TMPRSS2, using luciferase reporter virus HCoV-229E-Luc showed that luteolin exhibited a dose-dependent inhibition of infection. Respective IC50 values of 1.77 µM and 1.95 µM were determined. Under its glycosylated form (luteolin-7-O-glucoside), luteolin was inactive against HCoV-229E. Time of addition assay showed that utmost anti-HCoV-229E activity of luteolin was achieved when added at the post-inoculation step, indicating that luteolin acts as an inhibitor of the replication step of HCoV-229E. Unfortunately, no obvious antiviral activity for luteolin was found against SARS-CoV-2 and MERS-CoV in this study. In conclusion, luteolin isolated from Juncus acutus is a new inhibitor of alphacoronavirus HCoV-229E.Show less >

Language :

Anglais

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL