Title :

Comparison of two automated commercial assays for routine detection of anti-hepatitis E Virus IgM antibodies in clinical samples

Author(s) :

Becquart, Adrien [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Guigon, Aurelie [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Regueme, Alexandre [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Coulon, Pauline [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Bocket, Laurence [Auteur]
Laboratoire de virologie - ULR 3610
Hober, Didier [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Alidjinou, Enagnon Kazali [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]

Journal title :

Diagnostic Microbiology and Infectious Disease

Abbreviated title :

Diagn Microbiol Infect Dis

Volume number :

109

Pages :

116226

Publisher :

Elsevier

Publication date :

2024-02-20

ISSN :

1879-0070

English keyword(s) :

HEV
Diagnosis
IgM antibodies
Automated assays

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Diagnosis of hepatitis E virus (HEV) infection relies first on detection of IgM antibodies (Ab), sometimes completed with HEV RNA detection. This study aimed to compare the performance of two automated anti-HEV IgM Ab ...
Show more >Diagnosis of hepatitis E virus (HEV) infection relies first on detection of IgM antibodies (Ab), sometimes completed with HEV RNA detection. This study aimed to compare the performance of two automated anti-HEV IgM Ab assays. Correlation between Virclia® (Vircell) and Liaison® (Diasorin) assays was carried out on 178 routine clinical samples. Both assays were run on 67 samples from HEV RT-PCR (Altona) screened patients, and 52 Wantai® EIA (Euroimmun) tested samples. An excellent correlation was observed between both assays with an overall agreement of 96.6% (172/178), and a kappa coefficient at 0.93. In HEV RNA positive group (n=43), IgM detection rate was 93.3% (14/15) in immunocompetent patients, with both assays. In immunocompromised patients, detection rate was 75% (21/28) and 71.4% (20/28) using Virclia® and Liaison XL® assays, respectively. Virclia® and Liaison® anti-HEV IgM assays have similar performance for the detection of anti-HEV IgM Ab.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CHU Lille

Collections :

• Laboratoire de virologie - ULR 3610

Submission date :

2024-06-08T21:00:38Z
2024-09-27T09:11:52Z