Human mucin gene MUC5B, the 10.7-kb large ...
Document type :
Article dans une revue scientifique: Article original
DOI :
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Title :
Human mucin gene MUC5B, the 10.7-kb large central exon encodes various alternate subdomains resulting in a super-repeat. Structural evidence for a 11p15.5 gene family.
Author(s) :
Desseyn, Jean-Luc [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Guyonnet-Dupérat, V [Auteur]
Porchet, N [Auteur]
Aubert, J P [Auteur]
Laine, A [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Guyonnet-Dupérat, V [Auteur]
Porchet, N [Auteur]
Aubert, J P [Auteur]
Laine, A [Auteur]
Journal title :
THE JOURNAL OF BIOLOGICAL CHEMISTRY
Abbreviated title :
J Biol Chem
Volume number :
272
Pages :
3168-78
Publication date :
1997-02-07
ISSN :
0021-9258
English keyword(s) :
Amino Acid Sequence
Base Sequence
Blotting
Southern
DNA
Exons
Humans
Molecular Sequence Data
Mucin-5B
Mucins
Peptide Mapping
Repetitive Sequences
Nucleic Acid
Restriction Mapping
Sequence Alignment
Base Sequence
Blotting
Southern
DNA
Exons
Humans
Molecular Sequence Data
Mucin-5B
Mucins
Peptide Mapping
Repetitive Sequences
Nucleic Acid
Restriction Mapping
Sequence Alignment
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Human mucin gene MUC5B is mapped clustered with MUC6, MUC2, and MUC5AC on chromosome 11p15.5. We report here the isolation of three overlapping genomic clones of human MUC5B spanning approximately 40 kilobases. We have ...
Show more >Human mucin gene MUC5B is mapped clustered with MUC6, MUC2, and MUC5AC on chromosome 11p15.5. We report here the isolation of three overlapping genomic clones of human MUC5B spanning approximately 40 kilobases. We have determined their partial restriction maps and the intron-exon boundaries of the central region encoding a single open reading frame. This coding region has been completely sequenced. Its length is 10,713 base pairs, and it encodes a 3570-amino acid peptide. Nineteen subdomains have been individualized. Some subdomains show similarity to each other, creating larger composite repeat units that we have called super-repeats. Four super-repeats of 528 amino acid residues are thus observed within the central exon. Each comprises (i) a subdomain composed of 11 repeats of the irregular repeat of 29 amino acid residues, (ii) a unique conserved subdomain with no typical repeat, and (iii) a cysteine-rich subdomain. This latter subdomain has high sequence similarity to the cysteine-rich domains described in MUC2 and MUC5AC. Sequence data of these three genes, together with their clustered organization, lead us to suggest that they may be a part of a multigene family. The super-repeat present in MUC5B is the largest ever determined in mucin genes and the central exon of this gene is, by far, the largest reported for a vertebrate gene.Show less >
Show more >Human mucin gene MUC5B is mapped clustered with MUC6, MUC2, and MUC5AC on chromosome 11p15.5. We report here the isolation of three overlapping genomic clones of human MUC5B spanning approximately 40 kilobases. We have determined their partial restriction maps and the intron-exon boundaries of the central region encoding a single open reading frame. This coding region has been completely sequenced. Its length is 10,713 base pairs, and it encodes a 3570-amino acid peptide. Nineteen subdomains have been individualized. Some subdomains show similarity to each other, creating larger composite repeat units that we have called super-repeats. Four super-repeats of 528 amino acid residues are thus observed within the central exon. Each comprises (i) a subdomain composed of 11 repeats of the irregular repeat of 29 amino acid residues, (ii) a unique conserved subdomain with no typical repeat, and (iii) a cysteine-rich subdomain. This latter subdomain has high sequence similarity to the cysteine-rich domains described in MUC2 and MUC5AC. Sequence data of these three genes, together with their clustered organization, lead us to suggest that they may be a part of a multigene family. The super-repeat present in MUC5B is the largest ever determined in mucin genes and the central exon of this gene is, by far, the largest reported for a vertebrate gene.Show less >
Language :
Anglais
Audience :
Non spécifiée
Submission date :
2019-07-05T15:24:35Z
2019-07-08T13:48:02Z
2019-07-08T13:48:02Z
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