Cloning, chromosomal localization and ...
Document type :
Article dans une revue scientifique: Article original
PMID :
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Title :
Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4.
Author(s) :
Desseyn, Jean-Luc [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Clavereau, Isabelle [Auteur]
Laine, Anne [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Clavereau, Isabelle [Auteur]
Laine, Anne [Auteur]
Journal title :
European Journal of Biochemistry
Abbreviated title :
Eur J Biochem
Volume number :
269
Pages :
3150-9
Publication date :
2002-07-01
ISSN :
0014-2956
English keyword(s) :
Amino Acid Sequence
Animals
Chromosome Mapping
Chromosomes
Artificial
Bacterial
Cloning
Molecular
Conserved Sequence
Cosmids
Gene Expression Regulation
Humans
Intestinal Mucosa
Mice
Molecular Sequence Data
Mucin-4
Mucins
Organ Specificity
Rats
Repetitive Sequences
Amino Acid
Sequence Analysis
DNA
Sequence Homology
Amino Acid
Trachea
Animals
Chromosome Mapping
Chromosomes
Artificial
Bacterial
Cloning
Molecular
Conserved Sequence
Cosmids
Gene Expression Regulation
Humans
Intestinal Mucosa
Mice
Molecular Sequence Data
Mucin-4
Mucins
Organ Specificity
Rats
Repetitive Sequences
Amino Acid
Sequence Analysis
DNA
Sequence Homology
Amino Acid
Trachea
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
We report here the full coding sequence of a novel mouse putative membrane-associated mucin containing three extracellular EGF-like motifs and a mucin-like domain consisting of at least 20 tandem repeats of 124-126 amino ...
Show more >We report here the full coding sequence of a novel mouse putative membrane-associated mucin containing three extracellular EGF-like motifs and a mucin-like domain consisting of at least 20 tandem repeats of 124-126 amino acids. Screening a cosmid and a BAC libraries allowed to isolate several genomic clones. Genomic and cDNA sequence comparisons showed that the gene consists of 25 exons and 24 introns covering a genomic region of approximately 52 kb. The first intron is approximately 16 kb in length and is followed by an unusually large exon (approximately 9.5 kb) encoding Ser/Thr-rich tandemly repeated sequences. Radiation hybrid mapping localized this new gene to a mouse region of chromosome 16, which is the orthologous region of human chromosome 3q29 encompassing the large membrane-anchored mucin MUC4. Contigs analysis of the Human Genome Project did not reveal any other mucin on chromosome 3q29 and, interestingly, our analysis allowed the determination of the genomic organization of the human MUC4 and showed that its exon/intron structure is identical to that of the mouse gene we cloned. Furthermore, the human MUC4 shares considerable homologies with the mouse gene. Based on these data, we concluded that we isolated the mouse ortholog of MUC4 we propose as Muc4. Expression studies showed that Muc4 is ubiquitous like SMC and MUC4, with highest levels of expression in trachea and intestinal tract.Show less >
Show more >We report here the full coding sequence of a novel mouse putative membrane-associated mucin containing three extracellular EGF-like motifs and a mucin-like domain consisting of at least 20 tandem repeats of 124-126 amino acids. Screening a cosmid and a BAC libraries allowed to isolate several genomic clones. Genomic and cDNA sequence comparisons showed that the gene consists of 25 exons and 24 introns covering a genomic region of approximately 52 kb. The first intron is approximately 16 kb in length and is followed by an unusually large exon (approximately 9.5 kb) encoding Ser/Thr-rich tandemly repeated sequences. Radiation hybrid mapping localized this new gene to a mouse region of chromosome 16, which is the orthologous region of human chromosome 3q29 encompassing the large membrane-anchored mucin MUC4. Contigs analysis of the Human Genome Project did not reveal any other mucin on chromosome 3q29 and, interestingly, our analysis allowed the determination of the genomic organization of the human MUC4 and showed that its exon/intron structure is identical to that of the mouse gene we cloned. Furthermore, the human MUC4 shares considerable homologies with the mouse gene. Based on these data, we concluded that we isolated the mouse ortholog of MUC4 we propose as Muc4. Expression studies showed that Muc4 is ubiquitous like SMC and MUC4, with highest levels of expression in trachea and intestinal tract.Show less >
Language :
Anglais
Audience :
Non spécifiée
Submission date :
2019-07-05T15:27:17Z
2019-07-08T12:47:20Z
2019-07-08T12:47:20Z
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