Title :

Dietary (n-3) polyunsaturated fatty acids affect the kinetics of pro- and antiinflammatory responses in mice with Pseudomonas aeruginosa lung infection.

Author(s) :

Tiesset, Hélène [Auteur]
Pierre, Maud [Auteur]
Desseyn, Jean-Luc [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Guéry, Benoît [Auteur]
Beermann, Christopher [Auteur]
Galabert, Claude [Auteur]
gottrand, Fréderic [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Husson, Marie-Odile [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]

Journal title :

The Journal of Nutrition

Abbreviated title :

J Nutr

Volume number :

139

Pages :

82-9

Publication date :

2009-01-01

ISSN :

1541-6100

English keyword(s) :

Animals
Bronchoalveolar Lavage Fluid
Diet
Fatty Acids
Omega-3
Gene Expression Profiling
Gene Expression Regulation
Macrophages
Male
Mice
Mice
Inbred C57BL
Neurturin
Pneumonia
Bacterial
Pseudomonas Infections
Pseudomonas aeruginosa
Time

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The underlying mechanisms by which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) affect host resistance to Pseudomonas aeruginosa are unclear. The aim of this study was to determine their role on the kinetic ...
Show more >The underlying mechanisms by which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) affect host resistance to Pseudomonas aeruginosa are unclear. The aim of this study was to determine their role on the kinetic of pro- and antiinflammatory response in lung infection. Mice fed either a control diet or a diet enriched with EPA and DHA were infected intratracheally and we studied lung expression of proinflammatory markers [CXCL1, interleukin (IL)-6, tumor necrosis factor-alpha], antiinflammatory markers (IL-10, A20, and IkappaB alpha), and PPARalpha and PPARgamma. The inflammatory response was assessed using recruitment of neutrophils and macrophages into bronchoalveolar lavage fluid, bacterial clearance from the lung, pulmonary injury, and 7-d survival rate. Compared with the control group, EPA and DHA delayed the expression of proinflammatory markers during the first 2 h (P < 0.05), upregulated proinflammatory marker expression (P < 0.05), and induced overexpression of antiinflammatory markers at 8 h (P < 0.05), enhanced recruitment of neutrophils at 16 h (P < 0.05), and induced PPARalpha and PPARgamma overexpression at 4 and 8 h (P < 0.01), respectively. Pulmonary bacterial load decreased and pulmonary injury and mortality were reduced during the first 24 h (P < 0.05). In conclusion, EPA and DHA modulate the balance between pro- and antiinflammatory cytokines, alter the early response of the host to P. aeruginosa infection, and affect the early outcome of infection.Show less >

Language :

Anglais

Audience :

Non spécifiée

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2019-07-09T13:59:39Z
2019-07-10T07:08:00Z