A novel microRNA promotes coxsackievirus B4 infection of pancreatic β cells

Lalani, Salima; Knudsen, Joseph; Kenney, James; Hober, Didier; DiPersio, C. Michael; Gerber, Allen

Document type :

Article dans une revue scientifique: Article original

DOI :

10.3389/fimmu.2024.1414894

PMID :

39697323

Permalink :

http://hdl.handle.net/20.500.12210/126461

Title :

A novel microRNA promotes coxsackievirus B4 infection of pancreatic β cells

Author(s) :

Lalani, Salima [Auteur]
Albany Medical College
Knudsen, Joseph [Auteur]
Albany Medical College
Kenney, James [Auteur]
University at Albany [SUNY]
Hober, Didier [Auteur]

Laboratoire de virologie - ULR 3610
DiPersio, C. Michael [Auteur]
Albany Medical College
Gerber, Allen [Auteur]
Albany Medical College

Journal title :

Frontiers in Immunology

Abbreviated title :

Front. Immunol.

Volume number :

Publisher :

Frontiers

Publication date :

2024-12-04

ISSN :

1664-3224

English keyword(s) :

type 1 diabetes
coxsackievirus
microRNA
antiviral
pancreatic beta cells
trophoblast cells

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The epidemiological association of coxsackievirus B infection with type 1 diabetes suggests that therapeutic strategies that reduce viral load could delay or prevent disease onset. Moreover, recent studies suggest that ...
Show more >The epidemiological association of coxsackievirus B infection with type 1 diabetes suggests that therapeutic strategies that reduce viral load could delay or prevent disease onset. Moreover, recent studies suggest that treatment with antiviral agents against coxsackievirus B may help preserve insulin levels in type 1 diabetic patients. In the current study, we performed small RNA-sequencing to show that infection of immortalized trophoblast cells with coxsackievirus caused differential regulation of several miRNAs. One of these, hsa-miR-AMC1, was similarly upregulated in human pancreatic β cells infected with coxsackievirus B4. Moreover, treatment of β cells with non-cytotoxic concentrations of an antagomir that targets hsa-miR-AMC1 led to decreased CVB4 infection, suggesting a positive feedback loop wherein this microRNA further promotes viral infection. Interestingly, some predicted target genes of hsa-miR-AMC1 are shared with hsa-miR-184, a microRNA that is known to suppress genes that regulate insulin production in pancreatic β cells. Consistently, treatment of coxsackievirus B4-infected β cells with the hsa-miR-AMC1 antagomir was associated with a trend toward increased insulin production. Taken together, our findings implicate novel hsa-miR-AMC1 as a potential early biomarker of coxsackievirus B4-induced type 1 diabetes and suggest that inhibiting hsa-miR-AMC1 may provide therapeutic benefit to type 1 diabetes patients. Our findings also support the use of trophoblast cells as a model for identifying microRNAs that might be useful diagnostic markers or therapeutic targets for coxsackievirus B-induced type 1 diabetes.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CHU Lille

Collections :

Laboratoire de virologie - ULR 3610

Submission date :

2025-03-15T22:05:37Z
2025-03-27T15:55:44Z

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Except where otherwise noted, this item's license is described as Attribution 3.0 United States

Version	Link	Modification date
2	20.500.12210/126461*	2025-03-27T15:46:36Z
1	20.500.12210/126461.1	2025-03-15T22:05:37Z

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A novel microRNA promotes coxsackievirus ...

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