Isolation and structure elucidation of cell surface polysaccharides from Oenococcus oeni.

Maes, Emmanuel; Sadovskaya, Irina; Yamakawa, Nao; Goulet, A.; Le Marrec, C.; Chapot-Chartier, M. P.

Document type :

Article dans une revue scientifique: Article original

DOI :

10.1016/j.carres.2025.109456

PMID :

40112568

Permalink :

http://hdl.handle.net/20.500.12210/126715

Title :

Isolation and structure elucidation of cell surface polysaccharides from Oenococcus oeni.

Author(s) :

Maes, Emmanuel [Auteur]

Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Sadovskaya, Irina [Auteur]

BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Yamakawa, Nao [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Goulet, A. [Auteur]
Le Marrec, C. [Auteur]
Chapot-Chartier, M. P. [Auteur]

Journal title :

Carbohydrate Research

Abbreviated title :

Carbohydr Res

Volume number :

552

Pages :

109456

Publisher :

Elsevier

Publication date :

2025-03-31

ISSN :

1873-426X

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The Gram-positive bacterium Oenococcus oeni is a major player in wine malolactic fermentation. In O. oeni, cell wall polysaccharides are considered putative receptors for bacteriophages, virus predators that lead to ...
Show more >The Gram-positive bacterium Oenococcus oeni is a major player in wine malolactic fermentation. In O. oeni, cell wall polysaccharides are considered putative receptors for bacteriophages, virus predators that lead to fermentation failures. In this study, we have developed an efficient stepwise extraction protocol to extract polysaccharides from the cell wall of O. oeni IOEBS277, which were analyzed by methylation, 1D, 2D-NMR spectroscopy, and MALDI-QIT-TOF mass spectrometry. The chemical structures of the two major purified polysaccharides were elucidated. The first one is a heteropolysaccharide with repeating units consisting of a branched hexasaccharide and one glycerol residue, linked by phosphodiester bonds. The second one consists of a →6)-β-Galf-(1→ galactofuranan chain partially substituted on the C-2 hydroxyl with β-Glcp. HR-MAS NMR analysis of intact O. oeni cells indicated that both polysaccharides are exposed to the bacterial surface.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CHU Lille
CNRS
Inserm
Institut Pasteur de Lille

Collections :

Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41

Submission date :

2025-04-01T21:03:38Z
2025-04-09T07:42:59Z

Version	Link	Modification date
2	20.500.12210/126715*	2025-04-09T07:39:50Z
1	20.500.12210/126715.1	2025-04-01T21:03:38Z

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