Title :

Evaluation of mass spectrometry detection of pan-fungal serum disaccharide (MS-DS) for the diagnosis of invasive fungal infections: results from a collaborative study involving six European clinical centres.

Author(s) :

Cornu, Marjorie [Auteur]
Lille Inflammation Research International Center (LIRIC) - U995
Lille Inflammation Research International Center - U 995 [LIRIC]
Sendid, Boualem [Auteur]
Lille Inflammation Research International Center (LIRIC) - U995
Lille Inflammation Research International Center - U 995 [LIRIC]
Mery, Alexandre [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Francois, Nadine [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Malgorzata, Mikulska [Auteur]
Ospedale Policlinico San Martino [Genoa]
Letscher-Bru, Valerie [Auteur]
Université de Strasbourg [UNISTRA]
De Carolis, Elena [Auteur]
Fondazione Policlinico Universitario Agostino Gemelli IRCCS
Istituto di Ricovero e Cura a Carattere Scientifico [IRCCS]
Damonti, Lauro [Auteur]
Centre Hospitalier Universitaire Vaudois = Lausanne University Hospital [Lausanne] [CHUV]
Titecat, Marie [Auteur]
Lille Inflammation Research International Center (LIRIC) - U995
Lille Inflammation Research International Center - U 995 [LIRIC]
Bochud, Pierre-Yves [Auteur]
Centre Hospitalier Universitaire Vaudois = Lausanne University Hospital [Lausanne] [CHUV]
Alanio, Alexandre [Auteur]
Mycologie moléculaire
Université Paris Diderot - Paris 7 [UPD7]
Laboratoire de Parasitologie-Mycologie [CHU Saint Louis, Paris]
Sanguinetti, Maurizio [Auteur]
Fondazione Policlinico Universitario Agostino Gemelli IRCCS
Istituti di Ricovero e Cura a Carattere Scientifico [IRCCS]
Viscoli, Claudio [Auteur]
Ospedale Policlinico San Martino [Genoa]
Herbrecht, Raoul [Auteur]
Interface de Recherche Fondamentale et Appliquée en Cancérologie [IRFAC - Inserm U1113]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Poulain, Daniel [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]

Journal title :

Journal of clinical microbiology

Abbreviated title :

J. Clin. Microbiol.

Volume number :

57

Publication date :

2019-02-20

ISSN :

1098-660X

English keyword(s) :

Mucormycosis
serological diagnosis
mass spectrometry
invasive fungal infection
invasive aspergillosis
invasive candidiasis

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this ...
Show more >A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this assay. Patients with the following IFI were selected according to the availability of sera obtained at about the time that IFI was documented: invasive candidiasis (IC;  = 26 patients), invasive aspergillosis (IA;  = 19), and mucormycosis (MM;  = 23). Control sera originated from 20 neutropenic patients and 20 patients with bacteremia. MS-DS was carried out in blind manner for the diagnosis of IFI. A diagnosis of IC or IA was confirmed by detection of mannan (Man) or galactomannan (GM), respectively, associated with detection of (1,3)-β-d-glucan (BDG) in both infections. MM was detected by quantitative real-time PCR (qPCR). All tests discriminated sera from patients with IC from sera from control subjects with bacteremia (  ≤ 0.0009). For IC, the MS-DS sensitivity and specificity were 51% and 87%, respectively. MS-DS complemented the high specificity of Man monitoring. All tests discriminated sera from IA patients from sera from neutropenic controls (  ≤ 0.0009). For IA, MS-DS sensitivity and specificity were 64% and 95%, respectively. Only 13/36 serum samples from patients with MM were concordant by MS-DS and qPCR (6 were positive, and 7 were negative); 14 were positive by MS-DS alone. qPCR and MS-DS made a similar contribution to the diagnosis of MM. In patients undergoing long-term monitoring, the persistent circulation of serum disaccharide was observed, whereas DNA was detected only for a short period after initiation of treatment. MS-DS has an important role to play in the early diagnosis of IFI. Its panfungal nature and complementarity with other tests may justify its use in the management of IFI.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
CNRS
Inserm
Université de Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Submission date :

2019-10-22T14:20:53Z
2021-02-25T07:37:04Z
2021-11-03T10:27:26Z
2022-02-02T10:43:39Z