Alkynyl monosaccharide analogues as a tool ...
Type de document :
Article dans une revue scientifique
DOI :
PMID :
URL permanente :
Titre :
Alkynyl monosaccharide analogues as a tool for evaluating Golgi glycosylation efficiency: application to Congenital Disorders of Glycosylation (CDG)
Auteur(s) :
Vanbeselaere, Jorick [Auteur]
Vicogne, Dorothee [Auteur]
Matthijs, Gert [Auteur]
Biot, Christophe [Auteur]
Foulquier, Francois [Auteur]
Guerardel, Yann [Auteur]
Vicogne, Dorothee [Auteur]
Matthijs, Gert [Auteur]
Biot, Christophe [Auteur]
Foulquier, Francois [Auteur]
Guerardel, Yann [Auteur]
Titre de la revue :
Chemical Communications (Cambridge, England)
Nom court de la revue :
Chem. Commun. (Camb.)
Numéro :
49
Pagination :
11293-11295
Date de publication :
2013-12-14
ISSN :
1364-548X
Mot(s)-clé(s) en anglais :
Sialic Acid
Golgi Apparatus
Congenital Disorders of Glycosylation
Humans
Fibroblasts
Glycosylation
Fluorescent Dyes
Monosaccharides
Alkynes
Golgi Apparatus
Congenital Disorders of Glycosylation
Humans
Fibroblasts
Glycosylation
Fluorescent Dyes
Monosaccharides
Alkynes
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
The visualization of Golgi glycosylation defects in patients' cells with Congenital Disorders of Glycosylation (CDG) is challenging and necessitates the use of cumbersome glycan analysis methods that are barely adapted to ...
Lire la suite >The visualization of Golgi glycosylation defects in patients' cells with Congenital Disorders of Glycosylation (CDG) is challenging and necessitates the use of cumbersome glycan analysis methods that are barely adapted to clinical research. We show here that metabolic labelling of patient cells with alkyne-tagged sialic-acid (SiaNAl) enables an easy and reliable readout assay for the detection of CDG occurrence. It also provides valuable clues regarding the pathological processes by assessing the distribution of sialic acid analogues within the cells.Lire moins >
Lire la suite >The visualization of Golgi glycosylation defects in patients' cells with Congenital Disorders of Glycosylation (CDG) is challenging and necessitates the use of cumbersome glycan analysis methods that are barely adapted to clinical research. We show here that metabolic labelling of patient cells with alkyne-tagged sialic-acid (SiaNAl) enables an easy and reliable readout assay for the detection of CDG occurrence. It also provides valuable clues regarding the pathological processes by assessing the distribution of sialic acid analogues within the cells.Lire moins >
Langue :
Anglais
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Mécanismes moléculaires de la N-glycosylation et pathologies associées
Glycobiologie structurale des interactions hôtes-pathogènes
Glycobiologie structurale des interactions hôtes-pathogènes
Date de dépôt :
2020-02-12T15:11:09Z