Mycobacterium lutetiense sp. nov., ...
Document type :
Article dans une revue scientifique
DOI :
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Title :
Mycobacterium lutetiense sp. nov., Mycobacterium montmartrense sp. nov. and Mycobacterium arcueilense sp. nov., members of a novel group of non-pigmented rapidly growing mycobacteria recovered from a water distribution system
Author(s) :
Konjek, Julie [Auteur]
Infection et inflammation [2I]
Souded, Sabiha [Auteur]
Infection et inflammation [2I]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Trivelli, Xavier [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Bernut, Audrey [Auteur]
Centre d’études d’Agents Pathogènes et Biotechologies pour la Santé [CPBS]
Kremer, Laurent [Auteur]
Centre d’études d’Agents Pathogènes et Biotechologies pour la Santé [CPBS]
Welte, Benedicte [Auteur]
Eau de Paris
Joyeux, Michel [Auteur]
Eau de Paris
Dubrou, Sylvie [Auteur]
Euzeby, Jean-Paul [Auteur]
Gaillard, Jean-Louis [Auteur]
Infection et inflammation [2I]
Hôpital Ambroise Paré [AP-HP]
Sapriel, Guillaume [Auteur]
Infection et inflammation [2I]
Heym, Beate [Auteur]
Infection et inflammation [2I]
Hôpital Ambroise Paré [AP-HP]
Infection et inflammation [2I]
Souded, Sabiha [Auteur]
Infection et inflammation [2I]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Trivelli, Xavier [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Bernut, Audrey [Auteur]
Centre d’études d’Agents Pathogènes et Biotechologies pour la Santé [CPBS]
Kremer, Laurent [Auteur]
Centre d’études d’Agents Pathogènes et Biotechologies pour la Santé [CPBS]
Welte, Benedicte [Auteur]
Eau de Paris
Joyeux, Michel [Auteur]
Eau de Paris
Dubrou, Sylvie [Auteur]
Euzeby, Jean-Paul [Auteur]
Gaillard, Jean-Louis [Auteur]
Infection et inflammation [2I]
Hôpital Ambroise Paré [AP-HP]
Sapriel, Guillaume [Auteur]
Infection et inflammation [2I]
Heym, Beate [Auteur]
Infection et inflammation [2I]
Hôpital Ambroise Paré [AP-HP]
Journal title :
International Journal of Systematic and Evolutionary Microbiology
Abbreviated title :
Int. J. Syst. Evol. Microbiol.
Volume number :
66
Pages :
3694-3702
Publication date :
2016-09
ISSN :
1466-5034
English keyword(s) :
Rapidly growing mycobacteria
sp.nov
non tuberculous mycobacteria
molecular taxonomic analysis
biochemical analysis
rpoB-based identification
sp.nov
non tuberculous mycobacteria
molecular taxonomic analysis
biochemical analysis
rpoB-based identification
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
From our recent survey of non-pigmented rapidly growing mycobacteria in the Parisian water system, three groups of isolates (taxons 1-3) corresponding to possible novel species were selected for taxonomic study. The three ...
Show more >From our recent survey of non-pigmented rapidly growing mycobacteria in the Parisian water system, three groups of isolates (taxons 1-3) corresponding to possible novel species were selected for taxonomic study. The three taxa each formed creamy white, rough colonies, had an optimal growth temperature of 30 °C, hydrolyzed Tween 80, were catalase-positive at 22 °C and expressed arylsulfatase activity. All three were susceptible to amikacin, ciprofloxacin and tigecycline. The three taxa produced specific sets of mycolic acids, including one family that has never previously been described, as determined by thin layer chromatography and nuclear magnetic resonance. The partial rpoB sequences (723 bp) showed 4-6 % divergence from each other and more than 5 % differences from the most similar species. Partial 16S rRNA gene sequences showed 99 % identity within each species. The most similar sequences for 16S rRNA genes (98-99 % identity over 1444-1461 bp) were found in the Mycobacterium fortuitum group, Mycobacterium septicum and Mycobacterium farcinogenes. The three taxa formed a new clade (bootstrap value, 99 %) on trees reconstructed from concatenated partial 16S rRNA, hsp65 and rpoB sequences. The above results led us to propose three novel species for the three groups of isolates, namely Mycobacterium lutetiense sp. nov. [type strain 071T=ParisRGMnew_1T (CIP 110656T=DSM 46713T)], Mycobacterium montmartrense sp. nov. [type strain 196T=ParisRGMnew_2T (CIP 110655T=DSM 46714T)] and Mycobacteriu marcueilense sp. nov. [type strain of 269T=ParisRGMnew_3T (CIP 110654T=DSM 46715T)].Show less >
Show more >From our recent survey of non-pigmented rapidly growing mycobacteria in the Parisian water system, three groups of isolates (taxons 1-3) corresponding to possible novel species were selected for taxonomic study. The three taxa each formed creamy white, rough colonies, had an optimal growth temperature of 30 °C, hydrolyzed Tween 80, were catalase-positive at 22 °C and expressed arylsulfatase activity. All three were susceptible to amikacin, ciprofloxacin and tigecycline. The three taxa produced specific sets of mycolic acids, including one family that has never previously been described, as determined by thin layer chromatography and nuclear magnetic resonance. The partial rpoB sequences (723 bp) showed 4-6 % divergence from each other and more than 5 % differences from the most similar species. Partial 16S rRNA gene sequences showed 99 % identity within each species. The most similar sequences for 16S rRNA genes (98-99 % identity over 1444-1461 bp) were found in the Mycobacterium fortuitum group, Mycobacterium septicum and Mycobacterium farcinogenes. The three taxa formed a new clade (bootstrap value, 99 %) on trees reconstructed from concatenated partial 16S rRNA, hsp65 and rpoB sequences. The above results led us to propose three novel species for the three groups of isolates, namely Mycobacterium lutetiense sp. nov. [type strain 071T=ParisRGMnew_1T (CIP 110656T=DSM 46713T)], Mycobacterium montmartrense sp. nov. [type strain 196T=ParisRGMnew_2T (CIP 110655T=DSM 46714T)] and Mycobacteriu marcueilense sp. nov. [type strain of 269T=ParisRGMnew_3T (CIP 110654T=DSM 46715T)].Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Research team(s) :
Chemical Glycobiology
Submission date :
2020-02-12T15:11:12Z
2021-03-17T08:56:24Z
2021-03-17T08:56:24Z