Title :

DNA binding of the cell cycle transcriptional regulator GcrA depends on N6-adenosine methylation in Caulobacter crescentus and other Alphaproteobacteria

Author(s) :

Fioravanti, Antonella [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Fumeaux, Coralie [Auteur]
Université de Genève = University of Geneva [UNIGE]
Mohapatra, Saswat S. [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Bompard, Coralie [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Brilli, Matteo [Auteur]
Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 [LBBE]
Frandi, Antonio [Auteur]
Université de Genève = University of Geneva [UNIGE]
Castric, Vincent [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Villeret, Vincent [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Viollier, Patrick H. [Auteur]
Université de Genève = University of Geneva [UNIGE]
Biondi, Emanuele G. [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]

Journal title :

PLoS genetics

Abbreviated title :

PLoS Genet.

Volume number :

9

Pages :

e1003541

Publication date :

2013-05

ISSN :

1553-7404

English keyword(s) :

Alphaproteobacteria
Promoter Regions, Genetic
Amino Acid Sequence
Caulobacter crescentus
Adenosine
DNA Methylation
Methyltransferases
Epigenesis, Genetic
Transcription, Genetic
Gene Expression Regulation, Bacterial
Cell Cycle Proteins
DNA-Binding Proteins

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Several regulators are involved in the control of cell cycle progression in the bacterial model system Caulobacter crescentus, which divides asymmetrically into a vegetative G1-phase (swarmer) cell and a replicative S-phase ...
Show more >Several regulators are involved in the control of cell cycle progression in the bacterial model system Caulobacter crescentus, which divides asymmetrically into a vegetative G1-phase (swarmer) cell and a replicative S-phase (stalked) cell. Here we report a novel functional interaction between the enigmatic cell cycle regulator GcrA and the N6-adenosine methyltransferase CcrM, both highly conserved proteins among Alphaproteobacteria, that are activated early and at the end of S-phase, respectively. As no direct biochemical and regulatory relationship between GcrA and CcrM were known, we used a combination of ChIP (chromatin-immunoprecipitation), biochemical and biophysical experimentation, and genetics to show that GcrA is a dimeric DNA-binding protein that preferentially targets promoters harbouring CcrM methylation sites. After tracing CcrM-dependent N6-methyl-adenosine promoter marks at a genome-wide scale, we show that these marks recruit GcrA in vitro and in vivo. Moreover, we found that, in the presence of a methylated target, GcrA recruits the RNA polymerase to the promoter, consistent with its role in transcriptional activation. Since methylation-dependent DNA binding is also observed with GcrA orthologs from other Alphaproteobacteria, we conclude that GcrA is the founding member of a new and conserved class of transcriptional regulators that function as molecular effectors of a methylation-dependent (non-heritable) epigenetic switch that regulates gene expression during the cell cycle.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Évolution, Écologie et Paléontologie (Evo Eco Paleo) - UMR 8198

Research team(s) :

Biologie structurale et intégrative

Submission date :

2020-02-12T15:11:30Z
2021-03-11T14:46:55Z