Functions of maize genes encoding pyruvate ...
Document type :
Article dans une revue scientifique
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Title :
Functions of maize genes encoding pyruvate phosphate dikinase in developing endosperm
Author(s) :
Lappe, Ryan R. [Auteur]
Iowa State University [ISU]
Baier, John W. [Auteur]
University of Florida [Gainesville] [UF]
Boehlein, Susan K. [Auteur]
University of Florida [Gainesville] [UF]
Huffman, Ryan [Auteur]
Iowa State University [ISU]
Lin, Qiaohui [Auteur]
Iowa State University [ISU]
Wattebled, Fabrice [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Settles, A. Mark [Auteur]
University of Florida [Gainesville] [UF]
Hannah, L. Curtis [Auteur]
University of Florida [Gainesville] [UF]
Borisjuk, Ljudmilla [Auteur]
Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] [IPK-Gatersleben]
Rolletschek, Hardy [Auteur]
Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] [IPK-Gatersleben]
Stewart, Jon D. [Auteur]
University of Florida [Gainesville] [UF]
Scott, M. Paul [Auteur]
Iowa State University [ISU]
Hennen-Bierwagen, Tracie A. [Auteur]
Iowa State University [ISU]
Myers, Alan M. [Auteur]
Iowa State University [ISU]
Iowa State University [ISU]
Baier, John W. [Auteur]
University of Florida [Gainesville] [UF]
Boehlein, Susan K. [Auteur]
University of Florida [Gainesville] [UF]
Huffman, Ryan [Auteur]
Iowa State University [ISU]
Lin, Qiaohui [Auteur]
Iowa State University [ISU]
Wattebled, Fabrice [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Settles, A. Mark [Auteur]
University of Florida [Gainesville] [UF]
Hannah, L. Curtis [Auteur]
University of Florida [Gainesville] [UF]
Borisjuk, Ljudmilla [Auteur]
Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] [IPK-Gatersleben]
Rolletschek, Hardy [Auteur]
Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] [IPK-Gatersleben]
Stewart, Jon D. [Auteur]
University of Florida [Gainesville] [UF]
Scott, M. Paul [Auteur]
Iowa State University [ISU]
Hennen-Bierwagen, Tracie A. [Auteur]
Iowa State University [ISU]
Myers, Alan M. [Auteur]
Iowa State University [ISU]
Journal title :
Proceedings of the National Academy of Sciences of the United States of America
Abbreviated title :
Proc. Natl. Acad. Sci. U.S.A.
Volume number :
115
Pages :
E24-E33
Publication date :
2018-01
ISSN :
1091-6490
English keyword(s) :
Pyruvate, Orthophosphate Dikinase
PPDK
opaque
Zea mays
Energy Metabolism
Starch
Transcription Factors
Endosperm
metabolism
Plant Proteins
Mutation
maize
PPDK
opaque
Zea mays
Energy Metabolism
Starch
Transcription Factors
Endosperm
metabolism
Plant Proteins
Mutation
maize
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
Sciences du Vivant [q-bio]/Biologie végétale
Sciences du Vivant [q-bio]/Biologie végétale
English abstract : [en]
Maize opaque2 (o2) mutations are beneficial for endosperm nutritional quality but cause negative pleiotropic effects for reasons that are not fully understood. Direct targets of the bZIP transcriptional regulator encoded ...
Show more >Maize opaque2 (o2) mutations are beneficial for endosperm nutritional quality but cause negative pleiotropic effects for reasons that are not fully understood. Direct targets of the bZIP transcriptional regulator encoded by o2 include pdk1 and pdk2 that specify pyruvate phosphate dikinase (PPDK). This enzyme reversibly converts AMP, pyrophosphate, and phosphoenolpyruvate to ATP, orthophosphate, and pyruvate and provides diverse functions in plants. This study addressed PPDK function in maize starchy endosperm where it is highly abundant during grain fill. pdk1 and pdk2 were inactivated individually by transposon insertions, and both genes were simultaneously targeted by endosperm-specific RNAi. pdk2 accounts for the large majority of endosperm PPDK, whereas pdk1 specifies the abundant mesophyll form. The pdk1- mutation is seedling-lethal, indicating that C4 photosynthesis is essential in maize. RNAi expression in transgenic endosperm eliminated detectable PPDK protein and enzyme activity. Transgenic kernels weighed the same on average as nontransgenic siblings, with normal endosperm starch and total N contents, indicating that PPDK is not required for net storage compound synthesis. An opaque phenotype resulted from complete PPDK knockout, including loss of vitreous endosperm character similar to the phenotype conditioned by o2-. Concentrations of multiple glycolytic intermediates were elevated in transgenic endosperm, energy charge was altered, and starch granules were more numerous but smaller on average than normal. The data indicate that PPDK modulates endosperm metabolism, potentially through reversible adjustments to energy charge, and reveal that o2- mutations can affect the opaque phenotype through regulation of PPDK in addition to their previously demonstrated effects on storage protein gene expression.Show less >
Show more >Maize opaque2 (o2) mutations are beneficial for endosperm nutritional quality but cause negative pleiotropic effects for reasons that are not fully understood. Direct targets of the bZIP transcriptional regulator encoded by o2 include pdk1 and pdk2 that specify pyruvate phosphate dikinase (PPDK). This enzyme reversibly converts AMP, pyrophosphate, and phosphoenolpyruvate to ATP, orthophosphate, and pyruvate and provides diverse functions in plants. This study addressed PPDK function in maize starchy endosperm where it is highly abundant during grain fill. pdk1 and pdk2 were inactivated individually by transposon insertions, and both genes were simultaneously targeted by endosperm-specific RNAi. pdk2 accounts for the large majority of endosperm PPDK, whereas pdk1 specifies the abundant mesophyll form. The pdk1- mutation is seedling-lethal, indicating that C4 photosynthesis is essential in maize. RNAi expression in transgenic endosperm eliminated detectable PPDK protein and enzyme activity. Transgenic kernels weighed the same on average as nontransgenic siblings, with normal endosperm starch and total N contents, indicating that PPDK is not required for net storage compound synthesis. An opaque phenotype resulted from complete PPDK knockout, including loss of vitreous endosperm character similar to the phenotype conditioned by o2-. Concentrations of multiple glycolytic intermediates were elevated in transgenic endosperm, energy charge was altered, and starch granules were more numerous but smaller on average than normal. The data indicate that PPDK modulates endosperm metabolism, potentially through reversible adjustments to energy charge, and reveal that o2- mutations can affect the opaque phenotype through regulation of PPDK in addition to their previously demonstrated effects on storage protein gene expression.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Research team(s) :
Plant Storage Polysaccharides
Submission date :
2020-02-12T15:11:42Z
2021-03-04T09:44:05Z
2021-03-04T09:44:05Z