Title :

Insulin signaling controls the expression of O-GlcNAc transferase and its interaction with lipid microdomains

Author(s) :

Perez-Cervera, Yobana [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Universidad Nacional Autónoma de México = National Autonomous University of Mexico [UNAM]
Dehennaut, Vanessa [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Aquino-Gil, Moyira Osny [Auteur]
Universidad Nacional Autónoma de México = National Autonomous University of Mexico [UNAM]
Guedri, Katia [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Solórzano Mata, Carlos Josué [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Universidad Nacional Autónoma de México = National Autonomous University of Mexico [UNAM]
Olivier-Van Stichelen, Stéphanie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Michalski, Jean-Claude [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Foulquier, Francois [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Lefebvre, Tony [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]

Journal title :

The FASEB Journal

Abbreviated title :

FASEB J.

Volume number :

27

Pages :

3478-3486

Publication date :

2013-09

ISSN :

1530-6860

English keyword(s) :

lipid rafts
Signal Transduction
Humans
N-Acetylglucosaminyltransferases
Insulin
Phosphatidylinositol 3-Kinases
Blotting, Western
Cholesterol
Hep G2 Cells
Receptor, Insulin
PI3K pathway
Membrane Protein
OGT
Microscopy, Fluorescence

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Lipid microdomains (rafts) are cholesterol-enriched dynamic ordered lipid domains belonging to cell membranes involved in diverse cellular functions, including signal transduction, membrane trafficking, and infection. Many ...
Show more >Lipid microdomains (rafts) are cholesterol-enriched dynamic ordered lipid domains belonging to cell membranes involved in diverse cellular functions, including signal transduction, membrane trafficking, and infection. Many studies have reported relationships between insulin signaling and lipid rafts. Likewise, links between insulin signaling and O-GlcNAcylation have also been described. However, the potential connection between O-GlcNAc and raft dynamics remains unexplored. Here we show that O-GlcNAc and the enzyme that creates this modification, O-GlcNAc transferase (OGT), are localized in rafts. On insulin stimulation, we observe time-dependent increases in OGT expression and localization within rafts. We show that these processes depend on activation of the phosphatidylinositol 3-kinase (PI3K) pathway. Inhibition of OGT does not significantly affect cholesterol synthesis and raft building but decreases insulin receptor expression and PI3K and mitogen-activated protein kinase pathway activation. Taken together, these findings indicate that O-GlcNAcylation, lipid rafts, and signaling pathways are spatiotemporally coordinated to enable fundamental cellular functions.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Mécanismes de la Tumorigénèse et Thérapies Ciblées (M3T) - UMR 8161
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Mécanismes moléculaires de la N-glycosylation et pathologies associées
O-GlcNAcylation, signalisation cellulaire et cycle cellulaire

Submission date :

2020-02-12T15:11:50Z
2021-03-12T12:52:37Z