Title :

Characterization of Mucus-Related Properties of Streptococcus thermophilus: From Adhesion to Induction

Author(s) :

Fernandez, Neïké [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Wrzosek, Laura [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Radziwill-Bienkowska, Joanna M. [Auteur]
Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences [PAN]
Ringot-Destrez, Bélinda [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Duviau, Marie-Pierre [Auteur]
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP]
Noordine, Marie-Louise [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Laroute, Valérie [Auteur]
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP]
Robert, Véronique [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Cherbuy, Claire [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Daveran-Mingot, Marie-Line [Auteur]
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP]
Cocaign-Bousquet, Muriel [Auteur]
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP]
Leonard, Renaud [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Masselot, Catherine [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Rul, Françoise [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Ogier-Denis, Eric [Auteur]
Centre de recherche sur l'Inflammation [CRI (UMR_S_1149 / ERL_8252 / U1149)]
Thomas, Muriel [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Mercier-Bonin, Muriel [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]

Journal title :

Frontiers in physiology

Abbreviated title :

Front Physiol

Volume number :

9

Pages :

980

Publication date :

2018-07-24

ISSN :

1664-042X

English keyword(s) :

microbiota
mucin
gnotobiotic rodent
gut
Mucus
lactate
lactic acid bacteria

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Mucus is a major component of the intestinal barrier involved both in the protection of the host and the fitness of commensals of the gut. Streptococcus thermophilus is consumed world-wide in fermented dairy products and ...
Show more >Mucus is a major component of the intestinal barrier involved both in the protection of the host and the fitness of commensals of the gut. Streptococcus thermophilus is consumed world-wide in fermented dairy products and is also recognized as a probiotic, as its consumption is associated with improved lactose digestion. We determined the overall effect of S. thermophilus on the mucus by evaluating its ability to adhere, degrade, modify, or induce the production of mucus and/or mucins. Adhesion was analyzed in vitro using two types of mucins (from pig or human biopsies) and mucus-producing intestinal HT29-MTX cells. The induction of mucus was characterized in two different rodent models, in which S. thermophilus is the unique bacterial species in the digestive tract or transited as a sub-dominant bacterium through a complex microbiota. S. thermophilus LMD-9 and LMG18311 strains did not grow in sugars used to form mucins as the sole carbon source and displayed weak binding to mucus/mucins relative to the highly adhesive TIL448 Lactococcus lactis. The presence of S. thermophilus as the unique bacteria in the digestive tract of gnotobiotic rats led to accumulation of lactate and increased the number of Alcian-Blue positive goblet cells and the amount of the mucus-inducer KLF4 transcription factor. Lactate significantly increased KLF4 protein levels in HT29-MTX cells. Introduction of S. thermophilusvia transit as a sub-dominant bacterium (103 CFU/g feces) in a complex endogenous microbiota resulted in a slight increase in lactate levels in the digestive tract, no induction of overall mucus production, and moderate induction of sulfated mucin production. We thus show that although S. thermophilus is a poor mucus-adhesive bacterium, it can promote mucus pathway at least in part by producing lactate in the digestive tract.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Génétique des enveloppes bactériennes

Submission date :

2020-02-12T15:12:10Z
2021-03-12T13:40:55Z