Dual Effects of Hydrogen Sulfide Donor on ...
Document type :
Article dans une revue scientifique
Permalink :
Title :
Dual Effects of Hydrogen Sulfide Donor on Meiosis and Cumulus Expansion of Porcine Cumulus-Oocyte Complexes
Author(s) :
Nevoral, Jan [Auteur]
Petr, Jaroslav [Auteur]
Gelaude, Armance [Auteur]
Laboratoire de Régulation des Signaux de Division
Bodart, Jean-Francois [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Kučerová-Chrpová, Veronika [Auteur]
Sedmikova, Marketa [Auteur]
Krejčová, Tereza [Auteur]
Kolbabova, Tereza [Auteur]
Dvořáková, Markéta [Auteur]
Vyskocilova, Alena [Auteur]
Weingartová, Ivona [Auteur]
Krivohlavkova, Lenka [Auteur]
Žalmanová, Tereza [Auteur]
Jílek, František [Auteur]
Petr, Jaroslav [Auteur]
Gelaude, Armance [Auteur]
Laboratoire de Régulation des Signaux de Division
Bodart, Jean-Francois [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Kučerová-Chrpová, Veronika [Auteur]
Sedmikova, Marketa [Auteur]
Krejčová, Tereza [Auteur]
Kolbabova, Tereza [Auteur]
Dvořáková, Markéta [Auteur]
Vyskocilova, Alena [Auteur]
Weingartová, Ivona [Auteur]
Krivohlavkova, Lenka [Auteur]
Žalmanová, Tereza [Auteur]
Jílek, František [Auteur]
Journal title :
PLoS One
Volume number :
9
Pages :
e99613
Publication date :
2014-07-01
ISSN :
1932-6203
English keyword(s) :
Oocytes
Meiosis
MAPK signaling cascades
Metaphase
Histones
Vesicles
Pipettes
Hydrogen
Meiosis
MAPK signaling cascades
Metaphase
Histones
Vesicles
Pipettes
Hydrogen
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Hydrogen sulfide (H2S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H2S ...
Show more >Hydrogen sulfide (H2S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H2S acts on the meiotic maturation of porcine oocytes, including key maturation factors such as MPF and MAPK, and cumulus expansion intensity of cumulus-oocyte complexes. We observed that the H2S donor, Na2S, accelerated oocyte in vitro maturation in a dose-dependent manner, following an increase of MPF activity around germinal vesicle breakdown. Concurrently, the H2S donor affected cumulus expansion, monitored by hyaluronic acid production. Our results suggest that the H2S donor influences oocyte maturation and thus also participates in the regulation of cumulus expansion. The exogenous H2S donor apparently affects key signal pathways of oocyte maturation and cumulus expansion, resulting in faster oocyte maturation with little need of cumulus expansion.Show less >
Show more >Hydrogen sulfide (H2S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H2S acts on the meiotic maturation of porcine oocytes, including key maturation factors such as MPF and MAPK, and cumulus expansion intensity of cumulus-oocyte complexes. We observed that the H2S donor, Na2S, accelerated oocyte in vitro maturation in a dose-dependent manner, following an increase of MPF activity around germinal vesicle breakdown. Concurrently, the H2S donor affected cumulus expansion, monitored by hyaluronic acid production. Our results suggest that the H2S donor influences oocyte maturation and thus also participates in the regulation of cumulus expansion. The exogenous H2S donor apparently affects key signal pathways of oocyte maturation and cumulus expansion, resulting in faster oocyte maturation with little need of cumulus expansion.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Research team(s) :
Régulation des signaux de division
Submission date :
2020-02-12T15:44:32Z
2021-01-08T07:56:09Z
2021-01-08T08:02:22Z
2021-01-08T07:56:09Z
2021-01-08T08:02:22Z
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