Virus and cystic fibrosis: Rhinoviruses ...
Document type :
Compte-rendu et recension critique d'ouvrage
PMID :
Title :
Virus and cystic fibrosis: Rhinoviruses are associated with exacerbations in adult patients
Author(s) :
Goffard, Anne [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Lambert, Valérie [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Salleron, Julia [Auteur]
Institut de Cancérologie de Lorraine - Alexis Vautrin [Nancy] [UNICANCER/ICL]
Herwegh, Stéphanie [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Engelmann, Ilka [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Pinel, Claudine [Auteur]
Centre Hospitalier Universitaire [CHU Grenoble] [CHUGA]
Pin, Isabelle [Auteur]
Centre Hospitalier Universitaire [CHU Grenoble] [CHUGA]
Perrez, Thierry [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Prévotat, Anne [Auteur]
Hôpital Albert Calmette
Dewilde, Anny [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Delhaes, Laurence [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Lambert, Valérie [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Salleron, Julia [Auteur]
Institut de Cancérologie de Lorraine - Alexis Vautrin [Nancy] [UNICANCER/ICL]
Herwegh, Stéphanie [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Engelmann, Ilka [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Pinel, Claudine [Auteur]
Centre Hospitalier Universitaire [CHU Grenoble] [CHUGA]
Pin, Isabelle [Auteur]
Centre Hospitalier Universitaire [CHU Grenoble] [CHUGA]
Perrez, Thierry [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Prévotat, Anne [Auteur]
Hôpital Albert Calmette
Dewilde, Anny [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Delhaes, Laurence [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Journal title :
Journal of Clinical Virology
Pages :
147-153
Publisher :
Elsevier
Publication date :
2014-06
ISSN :
1386-6532
English keyword(s) :
PCR
Pulmonary exacerbation
Rhinovirus
Cystic fibrosis
Pulmonary exacerbation
Rhinovirus
Cystic fibrosis
HAL domain(s) :
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
Sciences du Vivant [q-bio]/Médecine humaine et pathologie
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
Sciences du Vivant [q-bio]/Médecine humaine et pathologie
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses
English abstract : [en]
BackgroundFew studies have suggested the potential role of respiratory viruses in cystic fibrosis (CF) exacerbation, but their real impact is probably underestimated.MethodSixty-four sputum samples collected from 46 adult ...
Show more >BackgroundFew studies have suggested the potential role of respiratory viruses in cystic fibrosis (CF) exacerbation, but their real impact is probably underestimated.MethodSixty-four sputum samples collected from 46 adult patients were included in the study: 33 samples were collected during exacerbation of CF, and 31 during the stable phase. After extraction, nucleic acids were tested for the presence of respiratory viruses. When rhinovirus (HRV) was detected, the 5′UTR and VP4/2 regions were sequenced, and phylogenetically analyzed. The characteristics of patients in exacerbation and stable phase were compared.ResultsViruses were found in 25% of samples. The HRV viruses were the most frequently detected followed by coronaviruses. Only the HRV detection was significantly associated with the occurrence of CF pulmonary exacerbation (p < 0.027). Characterization of 5′UTR and VP4/2 regions of the HRV genome specified that HRV-A, -B, -C were detected. All HRV-C were recombinant HRV-Ca.ConclusionsHRV were the most frequently detected viruses; their detection was significantly associated with the occurrence of an exacerbation. The reality of viral recombination between HRV was demonstrated in CF patients for the first time, raising the role of viruses in lung microbiota. Further studies are now warranted to decipher virus impact in CFShow less >
Show more >BackgroundFew studies have suggested the potential role of respiratory viruses in cystic fibrosis (CF) exacerbation, but their real impact is probably underestimated.MethodSixty-four sputum samples collected from 46 adult patients were included in the study: 33 samples were collected during exacerbation of CF, and 31 during the stable phase. After extraction, nucleic acids were tested for the presence of respiratory viruses. When rhinovirus (HRV) was detected, the 5′UTR and VP4/2 regions were sequenced, and phylogenetically analyzed. The characteristics of patients in exacerbation and stable phase were compared.ResultsViruses were found in 25% of samples. The HRV viruses were the most frequently detected followed by coronaviruses. Only the HRV detection was significantly associated with the occurrence of CF pulmonary exacerbation (p < 0.027). Characterization of 5′UTR and VP4/2 regions of the HRV genome specified that HRV-A, -B, -C were detected. All HRV-C were recombinant HRV-Ca.ConclusionsHRV were the most frequently detected viruses; their detection was significantly associated with the occurrence of an exacerbation. The reality of viral recombination between HRV was demonstrated in CF patients for the first time, raising the role of viruses in lung microbiota. Further studies are now warranted to decipher virus impact in CFShow less >
Language :
Anglais
Popular science :
Non
Source :
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