Title :

Growth Hormone (GH) Deficient Mice With GHRH Gene Ablation Are Severely Deficient in Vaccine and Immune Responses Against Streptococcus pneumoniae

Author(s) :

Farhat, Khalil [Auteur]
Université de Liège
Bodart, Gwennaëlle [Auteur]
Université de Liège
Charlet-Renard, Chantal [Auteur]
Université de Liège
Desmet, Christophe [Auteur]
Université de Liège
Moutschen, Michel [Auteur]
Université de Liège
Beguin, Yves [Auteur]
Université de Liège
Baron, Frederic [Auteur]
Université de Liège
Melin, Pierrette [Auteur]
Centre Hospitalier Universitaire de Liège [CHU-Liège]
Université de Liège
Quatresooz, Pascale [Auteur]
Université de Liège
Parent, Anne-Simone [Auteur]
Université de Liège
Desmecht, Daniel [Auteur]
Université de Liège
Sirard, Jean-Claude [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Salvatori, Roberto [Auteur]
Johns Hopkins University School of Medicine [Baltimore]
Martens, Henri [Auteur]
Université de Liège
Geenen, Vincent [Auteur correspondant]
Université de Liège

Journal title :

Frontiers in Immunology

Pages :

2175

Publisher :

Frontiers

Publication date :

2018-10-02

ISSN :

1664-3224

English keyword(s) :

thymo-independent antigen
spleen
somatotrope axis
GH
GHRH
S. pneumonia

HAL domain(s) :

Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Immunologie

English abstract : [en]

The precise impact of the somatotrope axis upon the immune system is still highly debated. We have previously shown that mice with generalized ablation of growth hormone (GH) releasing hormone (GHRH) gene (Ghrh -/-) have ...
Show more >The precise impact of the somatotrope axis upon the immune system is still highly debated. We have previously shown that mice with generalized ablation of growth hormone (GH) releasing hormone (GHRH) gene (Ghrh -/-) have normal thymus and T-cell development, but present a marked spleen atrophy and B-cell lymphopenia. Therefore, in this paper we have investigated vaccinal and anti-infectious responses of Ghrh -/- mice against S. pneumoniae, a pathogen carrying T-independent antigens. Ghrh -/- mice were unable to trigger production of specific IgM after vaccination with either native pneumococcal polysaccharides (PPS, PPV23) or protein-PPS conjugate (PCV13). GH supplementation of Ghrh -/- mice restored IgM response to PPV23 vaccine but not to PCV13 suggesting that GH could exert a specific impact on the spleen marginal zone that is strongly implicated in T-independent response against pneumococcal polysaccharides. As expected, after administration of low dose of S. pneumoniae, wild type (WT) completely cleared bacteria after 24 h. In marked contrast, Ghrh -/- mice exhibited a dramatic susceptibility to S. pneumoniae infection with a time-dependent increase in lung bacterial load and a lethal bacteraemia already after 24 h. Lungs of infected Ghrh -/- mice were massively infiltrated by inflammatory macrophages and neutrophils, while lung B cells were markedly decreased. The inflammatory transcripts signature was significantly elevated in Ghrh -/- mice. In this animal model, the somatotrope GHRH/GH/IGF1 axis plays a vital and unsuspected role in vaccine and immunological defense against S. pneumoniae.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL