Measuring Interactions between Fluorescent ...
Document type :
Article dans une revue scientifique
DOI :
Permalink :
Title :
Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
Author(s) :
Terryn, Christine [Auteur]
Plateforme en Imagerie Cellulaire et Tissulaire [PICT]
Habrant, Anouck [Auteur]
Fractionnement des AgroRessources et Environnement [FARE]
Paës, Gabriel [Auteur]
Fractionnement des AgroRessources et Environnement [FARE]
Spriet, Corentin [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Plateforme en Imagerie Cellulaire et Tissulaire [PICT]
Habrant, Anouck [Auteur]
Fractionnement des AgroRessources et Environnement [FARE]
Paës, Gabriel [Auteur]
Fractionnement des AgroRessources et Environnement [FARE]
Spriet, Corentin [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Journal title :
Journal of Visualized Experiments
Abbreviated title :
JoVE
Publisher :
MyJove Corporation
Publication date :
2020-01-02
ISSN :
1940-087X
English keyword(s) :
Biochemistry
Issue 155
sFLIM
Fluorescence lifetime
FRET
Autofluorescence
Lignocellulose
Interaction
Issue 155
sFLIM
Fluorescence lifetime
FRET
Autofluorescence
Lignocellulose
Interaction
HAL domain(s) :
Chimie
English abstract : [en]
In lignocellulosic biomass (LB), the activity of enzymes is limited by the appearance of non-specific interactions with lignin during the hydrolysisprocess, which maintains enzymes far from their substrate. Characterization ...
Show more >In lignocellulosic biomass (LB), the activity of enzymes is limited by the appearance of non-specific interactions with lignin during the hydrolysisprocess, which maintains enzymes far from their substrate. Characterization of these complex interactions is thus a challenge in complexsubstrates such as LB. The method here measures molecular interactions between fluorophore-tagged molecules and native autofluorescentlignin, to be revealed by Förster resonance energy transfer (FRET). Contrary to FRET measurements in living cells using two exogenousfluorophores, FRET measurements in plants using lignin is not trivial due to its complex autofluorescence. We have developed an originalacquisition and analysis pipeline with correlated observation of two complementary properties of fluorescence: fluorescence emission andlifetime. sFLIM (spectral and fluorescent lifetime imaging microscopy) provides the quantification of these interactions with high sensitivity,revealing different interaction levels between biomolecules and lignin.Show less >
Show more >In lignocellulosic biomass (LB), the activity of enzymes is limited by the appearance of non-specific interactions with lignin during the hydrolysisprocess, which maintains enzymes far from their substrate. Characterization of these complex interactions is thus a challenge in complexsubstrates such as LB. The method here measures molecular interactions between fluorophore-tagged molecules and native autofluorescentlignin, to be revealed by Förster resonance energy transfer (FRET). Contrary to FRET measurements in living cells using two exogenousfluorophores, FRET measurements in plants using lignin is not trivial due to its complex autofluorescence. We have developed an originalacquisition and analysis pipeline with correlated observation of two complementary properties of fluorescence: fluorescence emission andlifetime. sFLIM (spectral and fluorescent lifetime imaging microscopy) provides the quantification of these interactions with high sensitivity,revealing different interaction levels between biomolecules and lignin.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Non spécifiée
ANR Project :
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Research platform(s) :
Traitement de l'image et du signal pour la biologie (TISBio)
Submission date :
2020-07-06T14:32:48Z
2020-09-28T10:34:18Z
2020-09-28T10:34:18Z
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