Title :

Colorimetric sensing of dopamine in beef meat using copper sulfide encapsulated within bovine serum albumin functionalized with copper phosphate (CuS-BSA-Cu3(PO4)2) nanoparticles

Author(s) :

Swaidan, Abir [Auteur]

Barras, Alexandre [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Addad, Ahmed [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Unité Matériaux et Transformations - UMR 8207 [UMET]
Tahon, Jean-Francois [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Unité Matériaux et Transformations - UMR 8207 [UMET]
Toufaily, Joumana [Auteur]

Hamieh, Tayssir [Auteur]

Szunerits, Sabine [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Boukherroub, Rabah [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]

Journal title :

Journal of Colloid and Interface Science

Abbreviated title :

Journal of Colloid and Interface Science

Pages :

732-740

Publisher :

Elsevier BV

Publication date :

2021-01

ISSN :

0021-9797

English keyword(s) :

CuS-BSA-Cu3(PO4)2
Nanozymes
Colorimetric
Detection
Dopamine
Beef meat

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

A novel nanosensor with peroxidase enzyme-mimetic activity, based on CuS-BSA-Cu3(PO4)2 nanoparticles, was developed. CuS-BSA nanoparticles were first synthesized using a facile bio-mineralization assay. Conjugation of ...
Show more >A novel nanosensor with peroxidase enzyme-mimetic activity, based on CuS-BSA-Cu3(PO4)2 nanoparticles, was developed. CuS-BSA nanoparticles were first synthesized using a facile bio-mineralization assay. Conjugation of Cu3(PO4)2 with CuS-BSA generates CuS-BSA-Cu3(PO4)2 nanoparticles (NPs) of 10 nm in size with high catalytic activity against a peroxidase substrate, 3,3′,5,5′-tetramethylbenzidine (TMB). The catalytic action was based on a remarkable color change from colorless TMB into blue oxidized product (oxTMB) with absorption maximum at 654 nm. The enzyme-mimetic activity of CuS-BSA-Cu3(PO4)2 nanoparticles was believed to occur through hydroxyl radical (HOradical dot) generation in presence of H2O2, which was inhibited upon addition of dopamine. Increasing concentrations of dopamine induced a gradual decrease of the nanoparticles’ catalytic activity. The developed colorimetric sensor displayed a limit of detection of 0.13 µM for dopamine over 0.05–100 µM linear range and high specificity. The performance of the nanosensor for sensing dopamine in beef meat and blood samples was evaluated and proved to be promising for diagnostic applications without the requirement of complex and expensive instrumentation.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

Université de Lille
CNRS
INRA
ENSCL

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Submission date :

2020-11-05T16:27:24Z
2020-11-17T09:04:10Z