The Genes Encode an Original Legionaminic ...
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Article dans une revue scientifique
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Title :
The Genes Encode an Original Legionaminic Acid Pathway Required for Crust Assembly in Bacillus subtilis.
Author(s) :
Dubois, Thomas [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Krzewinski, Frederic [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Yamakawa, Nao [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Lemy, Christelle [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Hamiot, Audrey [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
BRUNET, Loïc [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Lacoste, Anne-Sophie [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Knirel, Yuryi [Auteur]
ND Zelinsky Institute of Organic Chemistry [Moscow, Russia]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Faille, Christine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Krzewinski, Frederic [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Yamakawa, Nao [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Lemy, Christelle [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Hamiot, Audrey [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
BRUNET, Loïc [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Lacoste, Anne-Sophie [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Knirel, Yuryi [Auteur]
ND Zelinsky Institute of Organic Chemistry [Moscow, Russia]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Faille, Christine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Journal title :
mBio
Abbreviated title :
mBio
Volume number :
11
Publication date :
2020-08-18
ISSN :
2150-7511
English keyword(s) :
Bacillus subtilis
bacterial adhesion
crust
legionaminic acid
nonulosonic acid
spores
bacterial adhesion
crust
legionaminic acid
nonulosonic acid
spores
HAL domain(s) :
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]
English abstract : [en]
The crust is the outermost spore layer of most strains devoid of an exosporium. This outermost layer, composed of both proteins and carbohydrates, plays a major role in the adhesion and spreading of spores into the ...
Show more >The crust is the outermost spore layer of most strains devoid of an exosporium. This outermost layer, composed of both proteins and carbohydrates, plays a major role in the adhesion and spreading of spores into the environment. Recent studies have identified several crust proteins and have provided insights about their organization at the spore surface. However, although carbohydrates are known to participate in adhesion, little is known about their composition, structure, and localization. In this study, we showed that the spore surface of is covered with legionaminic acid (Leg), a nine-carbon backbone nonulosonic acid known to decorate the flagellin of the human pathogens and We demonstrated that the , , , , and genes of are required for Leg biosynthesis during sporulation, while the gene is required for Leg transfer from the mother cell to the surface of the forespore. We also characterized the activity of SpsM and highlighted an original Leg biosynthesis pathway in Finally, we demonstrated that Leg is required for the assembly of the crust around the spores, and we showed that in the absence of Leg, spores were more adherent to stainless steel probably because of their reduced hydrophilicity and charge. species are a major economic and food safety concern of the food industry because of their food spoilage-causing capability and persistence. Their persistence is mainly due to their ability to form highly resistant spores adhering to the surfaces of industrial equipment. Spores of the group are surrounded by the crust, a superficial layer which plays a key role in their adhesion properties. However, knowledge of the composition and structure of this layer remains incomplete. Here, for the first time, we identified a nonulosonic acid (Leg) at the surfaces of bacterial spores (). We uncovered a novel Leg biosynthesis pathway, and we demonstrated that Leg is required for proper crust assembly. This work contributes to the description of the structure and composition of spores which has been under way for decades, and it provides keys to understanding the importance of carbohydrates in adhesion and persistence in the food industry.Show less >
Show more >The crust is the outermost spore layer of most strains devoid of an exosporium. This outermost layer, composed of both proteins and carbohydrates, plays a major role in the adhesion and spreading of spores into the environment. Recent studies have identified several crust proteins and have provided insights about their organization at the spore surface. However, although carbohydrates are known to participate in adhesion, little is known about their composition, structure, and localization. In this study, we showed that the spore surface of is covered with legionaminic acid (Leg), a nine-carbon backbone nonulosonic acid known to decorate the flagellin of the human pathogens and We demonstrated that the , , , , and genes of are required for Leg biosynthesis during sporulation, while the gene is required for Leg transfer from the mother cell to the surface of the forespore. We also characterized the activity of SpsM and highlighted an original Leg biosynthesis pathway in Finally, we demonstrated that Leg is required for the assembly of the crust around the spores, and we showed that in the absence of Leg, spores were more adherent to stainless steel probably because of their reduced hydrophilicity and charge. species are a major economic and food safety concern of the food industry because of their food spoilage-causing capability and persistence. Their persistence is mainly due to their ability to form highly resistant spores adhering to the surfaces of industrial equipment. Spores of the group are surrounded by the crust, a superficial layer which plays a key role in their adhesion properties. However, knowledge of the composition and structure of this layer remains incomplete. Here, for the first time, we identified a nonulosonic acid (Leg) at the surfaces of bacterial spores (). We uncovered a novel Leg biosynthesis pathway, and we demonstrated that Leg is required for proper crust assembly. This work contributes to the description of the structure and composition of spores which has been under way for decades, and it provides keys to understanding the importance of carbohydrates in adhesion and persistence in the food industry.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Research team(s) :
Glycobiologie structurale des interactions hôtes-pathogènes
Submission date :
2020-11-18T14:25:38Z
2020-11-19T12:04:13Z
2020-11-19T12:05:04Z
2020-11-19T12:11:50Z
2020-11-19T12:04:13Z
2020-11-19T12:05:04Z
2020-11-19T12:11:50Z
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