GlcNAc6ST3 is a keratan sulfate sulfotransferase ...
Document type :
Article dans une revue scientifique
Permalink :
Title :
GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain
Author(s) :
Narentuya, null [Auteur]
Uchimura, Yoshiko [Auteur]
Nagoya University
Foyez, Tahmina [Auteur]
Zhang, Zui [Auteur]
Akama, Tomoya O. [Auteur]
Yagi, Hirokazu [Auteur]
Kato, Koichi [Auteur]
Komatsu, Yukio [Auteur]
Kadomatsu, Kenji [Auteur]
Uchimura, Kenji [Auteur]
Nagoya University
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Uchimura, Yoshiko [Auteur]
Nagoya University
Foyez, Tahmina [Auteur]
Zhang, Zui [Auteur]
Akama, Tomoya O. [Auteur]
Yagi, Hirokazu [Auteur]
Kato, Koichi [Auteur]
Komatsu, Yukio [Auteur]
Kadomatsu, Kenji [Auteur]
Uchimura, Kenji [Auteur]
Nagoya University
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Journal title :
Scientific Reports
Abbreviated title :
Sci Rep
Volume number :
9
Publisher :
Springer Nature
Publication date :
2019-03-13
ISSN :
2045-2322
HAL domain(s) :
Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Keratan sulfate (KS) is a carbohydrate side chain covalently attached to extracellular proteoglycans. KS is composed of disaccharide units of 6-sulfated N-acetylglucosamine (GlcNAc) and galactose. We have previously shown ...
Show more >Keratan sulfate (KS) is a carbohydrate side chain covalently attached to extracellular proteoglycans. KS is composed of disaccharide units of 6-sulfated N-acetylglucosamine (GlcNAc) and galactose. We have previously shown that GlcNAc-6-O-sulfotransferase (GlcNAc6ST) 1 encoded by Chst2 is an enzyme necessary for the synthesis of GlcNAc-6-sulfated KS chains that are required for neuronal plasticity in the visual cortex of the mouse brain during the critical period, but not in adulthood. Here, we show that GlcNAc-6-sulfated KS recognized by the R-10G anti-KS antibody, of which the minimum epitope structure is Galß1-4GlcNAc(6S)ß1-3Galß1-4GlcNAc(6S), distributes diffusely in neuropils and presents densely in close proximity to the perineuronal region of the perineuronal net (PNN)-positive neurons in the adult visual cortex. Surprisingly, GlcNAc6ST3, which was discovered as an intestinal GlcNAc6ST encoded by Chst5, is a major brain KS sulfotransferase expressed in oligodendrocytes in adulthood. Moreover, we identified an isoform of the protein-tyrosine phosphatase PTPRZ as a R-10G-reactive KS proteoglycan. These results indicate that GlcNAc6ST3 may play a role in synthesis of a component of PNN in the adult brain, and that the KS-modified isoform of PTPRZ encoded by Ptprz1 could be an extracellular molecule associated with PNNs.Show less >
Show more >Keratan sulfate (KS) is a carbohydrate side chain covalently attached to extracellular proteoglycans. KS is composed of disaccharide units of 6-sulfated N-acetylglucosamine (GlcNAc) and galactose. We have previously shown that GlcNAc-6-O-sulfotransferase (GlcNAc6ST) 1 encoded by Chst2 is an enzyme necessary for the synthesis of GlcNAc-6-sulfated KS chains that are required for neuronal plasticity in the visual cortex of the mouse brain during the critical period, but not in adulthood. Here, we show that GlcNAc-6-sulfated KS recognized by the R-10G anti-KS antibody, of which the minimum epitope structure is Galß1-4GlcNAc(6S)ß1-3Galß1-4GlcNAc(6S), distributes diffusely in neuropils and presents densely in close proximity to the perineuronal region of the perineuronal net (PNN)-positive neurons in the adult visual cortex. Surprisingly, GlcNAc6ST3, which was discovered as an intestinal GlcNAc6ST encoded by Chst5, is a major brain KS sulfotransferase expressed in oligodendrocytes in adulthood. Moreover, we identified an isoform of the protein-tyrosine phosphatase PTPRZ as a R-10G-reactive KS proteoglycan. These results indicate that GlcNAc6ST3 may play a role in synthesis of a component of PNN in the adult brain, and that the KS-modified isoform of PTPRZ encoded by Ptprz1 could be an extracellular molecule associated with PNNs.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Research team(s) :
Diversité structurale des héparanes sulfates et régulation de la réponse inflammatoire
Submission date :
2020-12-14T10:28:41Z
2020-12-17T09:39:34Z
2020-12-17T09:42:08Z
2020-12-17T09:39:34Z
2020-12-17T09:42:08Z
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