Title :

Structural and Proteomic Changes in Viable but Non-culturable Vibrio cholerae

Author(s) :

Brenzinger, Susanne [Auteur]

van der Aart, Lizah T. [Auteur]

van Wezel, Gilles P. [Auteur]

Lacroix, Jean-Marie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Glatter, Timo [Auteur]
Max Planck Institute for Terrestrial Microbiology
Briegel, Ariane [Auteur]

Journal title :

Frontiers in Microbiology

Abbreviated title :

Front. Microbiol.

Volume number :

10

Publisher :

Frontiers Media SA

Publication date :

2019-04-17

ISSN :

1664-302X

English keyword(s) :

Vibrio cholerae
viable but non-culturable
proteomics
electron cryotomography
cell envelope
bacterial ultrastructure

HAL domain(s) :

Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique

English abstract : [en]

Aquatic environments are reservoirs of the human pathogenVibrio choleraeO1, whichcauses the acute diarrheal disease cholera. Upon low temperature or limited nutrientavailability, the cells enter a viable but non-culturable ...
Show more >Aquatic environments are reservoirs of the human pathogenVibrio choleraeO1, whichcauses the acute diarrheal disease cholera. Upon low temperature or limited nutrientavailability, the cells enter a viable but non-culturable (VBNC) state. Characteristic of thisstate are an altered morphology, low metabolic activity, and lack of growth under standardlaboratory conditions. Here, for the first time, the cellular ultrastructure ofV. choleraeVBNC cells raised in natural waters was investigated using electron cryo-tomography.This was complemented by a comparison of the proteomes and the peptidoglycancomposition ofV. choleraefrom LB overnight cultures and VBNC cells. The extensiveremodeling of the VBNC cells was most obvious in the passive dehiscence of the cellenvelope, resulting in improper embedment of flagella and pili. Only minor changes of thepeptidoglycan and osmoregulated periplasmic glucans wereobserved. Active changesin VBNC cells included the production of cluster I chemosensory arrays and changeof abundance of cluster II array proteins. Components involved in iron acquisition andstorage, peptide import and arginine biosynthesis were overrepresented in VBNC cells,while enzymes of the central carbon metabolism were found atlower levels. Finally,several pathogenicity factors ofV. choleraewere less abundant in the VBNC state,potentially limiting their infectious potential. This study gives unprecedented insight intothe physiology of VBNC cells and the drastically altered presence of their metabolic andstructural proteins.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Génétique des enveloppes bactériennes

Submission date :

2020-12-18T14:04:41Z
2021-01-06T09:11:06Z