Entrapment of uropathogenic E. coli cells ...
Document type :
Article dans une revue scientifique
Permalink :
Title :
Entrapment of uropathogenic E. coli cells into ultra-thin sol-gel matrices on gold thin films: A low cost alternative for impedimetric bacteria sensing
Author(s) :
Jafari, Hamed [Auteur]
Amiri, Mandana [Auteur]
Abdi, Esmat [Auteur]
Navid, Saeid Latifi [Auteur]
Bouckaert, Julie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Jijie, Roxana [Auteur]
Boukherroub, Rabah [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Szunerits, Sabine [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Amiri, Mandana [Auteur]
Abdi, Esmat [Auteur]
Navid, Saeid Latifi [Auteur]
Bouckaert, Julie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Jijie, Roxana [Auteur]
Boukherroub, Rabah [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Szunerits, Sabine [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Journal title :
Biosensors and Bioelectronics
Volume number :
124-125
Pages :
161-166
Publisher :
Elsevier BV
Publication date :
2019-01-15
ISSN :
0956-5663
English keyword(s) :
Uropathogenic E. coli
Sol-gel
Thin film
Sensing
Electrochemical impedance spectroscopy
Sol-gel
Thin film
Sensing
Electrochemical impedance spectroscopy
HAL domain(s) :
Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Bacterial infections are causing worldwide morbidity and mortality. One way to limit infectious outbreaks and optimize clinical management of infections is through the development of fast and sensitive sensing of bacteria. ...
Show more >Bacterial infections are causing worldwide morbidity and mortality. One way to limit infectious outbreaks and optimize clinical management of infections is through the development of fast and sensitive sensing of bacteria. Most sensing approaches are currently based on immunological detection principles. We report here on an impedimetric sensor to selectively and sensitive detect uropathogenic E. coli cells (E. coli UTI89) using artificial recognition sites. We show here the possibility to imprint the rod-shape structure of E. coli UTI 89 into ultra-thin inorganic silica coatings on gold electrodes in a reproducible manner. A linear range from to 1 × 100 –1 × 104 cfu mL−1 is obtained. With a detection limit for E. coli UTI89 below 1 cfu mL−1 from five blank signals (95% confidence level) and excellent selective binding capabilities, these bacterial cell imprinted electrodes brings us closer to a low cost specific bacterial recognition surfaces.Show less >
Show more >Bacterial infections are causing worldwide morbidity and mortality. One way to limit infectious outbreaks and optimize clinical management of infections is through the development of fast and sensitive sensing of bacteria. Most sensing approaches are currently based on immunological detection principles. We report here on an impedimetric sensor to selectively and sensitive detect uropathogenic E. coli cells (E. coli UTI89) using artificial recognition sites. We show here the possibility to imprint the rod-shape structure of E. coli UTI 89 into ultra-thin inorganic silica coatings on gold electrodes in a reproducible manner. A linear range from to 1 × 100 –1 × 104 cfu mL−1 is obtained. With a detection limit for E. coli UTI89 below 1 cfu mL−1 from five blank signals (95% confidence level) and excellent selective binding capabilities, these bacterial cell imprinted electrodes brings us closer to a low cost specific bacterial recognition surfaces.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Collections :
Research team(s) :
Computational Molecular Systems Biology
Submission date :
2021-01-04T09:04:29Z
2021-01-07T11:50:21Z
2021-01-07T11:50:21Z
Files
- P19.95 jafari2019.pdf
- Version finale acceptée pour publication (postprint)
- Open access
- Access the document