Title :

Improved photodynamic effect through encapsulation of two photosensitizers in lipid nanocapsules

Author(s) :

Barras, Alexandre [Auteur correspondant]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Skandrani, Nadia [Auteur]
Nanomédecine, imagerie, thérapeutique - UFC (UR 4662) [NIT / NANOMEDECINE]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Gonzalez Pisfil, Mariano [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Paryzhak, Solomiya [Auteur]
Dumych, Tetiana [Auteur]
Haustrate, Aurélien [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Héliot, Laurent [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Gharbi, Tijani [Auteur]
Centre Hospitalier Régional Universitaire de Besançon [CHRU Besançon]
Nanomédecine, imagerie, thérapeutique - UFC (UR 4662) [NIT / NANOMEDECINE]
Boulahdour, Hatem [Auteur]
Centre Hospitalier Régional Universitaire de Besançon [CHRU Besançon]
Nanomédecine, imagerie, thérapeutique - UFC (UR 4662) [NIT / NANOMEDECINE]
Lehen'Kyi, V'Yacheslav [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Bilyy, Rostyslav [Auteur]
Szunerits, Sabine [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Bidaux, Gabriel [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Boukherroub, Rabah [Auteur correspondant]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]

Journal title :

Journal of materials chemistry‎ B

Pages :

5949 - 5963

Publisher :

Royal Society of Chemistry

Publication date :

2018

ISSN :

2050-750X

HAL domain(s) :

Chimie/Chimie thérapeutique
Sciences du Vivant [q-bio]/Sciences pharmaceutiques
Sciences du Vivant [q-bio]/Biologie cellulaire

English abstract : [en]

Photodynamic therapy (PDT) has developed into a new clinical and non-invasive treatment for cancer over the past 30 years. By the combination of three non-toxic partners, i.e. a photosensitizer (PS), molecular oxygen (O2) ...
Show more >Photodynamic therapy (PDT) has developed into a new clinical and non-invasive treatment for cancer over the past 30 years. By the combination of three non-toxic partners, i.e. a photosensitizer (PS), molecular oxygen (O2) and light, cytotoxic reactive oxygen species (ROS) are locally produced leading to irreversible vascular and cellular damage. In the present study, we report for the first time that the combination of two photosensitizers (2 PSs: Protoporphyrin IX, PpIX and Hypericin, Hy) loaded in the same lipid nanocapsules (LNCs) leads to enhanced photodynamic therapy efficiency when compared with previously reported systems. The 2 PS-loaded LNCs are shown to increase the in vitro phototoxicity at the nanomolar range (IC50 = 274 and 278 nM on HeLa and MDA-MB-231 cell lines, respectively), whereas the corresponding single PS-loaded LNCs at the same concentration exhibit a phototoxicity two times lower. Intracellular localization in HeLa cells indicates a subcellular asymmetry of PpIX and Hy, in the plasma, ER membranes and round internal structures. The biodistribution of LNCs was studied upon different routes of injection into Swiss nude mice; based on the obtained data, LNCs were injected intratumorally and used to slow the growth of xenograft tumors in mice. The results obtained in this study suggest that the combination of two or more PSs may be a promising strategy to improve the efficacy of conventional photodynamic therapy as well as to reduce dark toxicity.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

ANR Project :

Canaux ioniques d'intérêt thérapeutique

Collections :

• Laboratoire de Physique des Lasers, Atomes et Molécules (PhLAM) - UMR 8523

Source :

Harvested from HAL